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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

1-(Carboxymethylthio)tetradecane attenuates PDGF- and TPA-induced c-fos mRNA expression and increases the formation of phosphatidylethanolamine with a shift from less to more polar molecular species in C3H/10T1/2 cells.

1-(Carboxymethylthio)tetradecane caused C3H/10T1/2Cl8 and C3H/10T1/2Cl16 to incorporate 10 times more [32P]Pi into diacylphosphatidylethanolamine than control. This 3-thia fatty acid caused a shift in incorporation of 32P-radioactivity into phosphatidylethanolamine species from species with long to species with short HPLC elution times. The increase in 32P-labeling was parallelled by a change in the apparent mass of phosphatidylethanolamine to a higher proportion of molecular species with short elution times than with long elution times. 1-(Carboxymethylthio)tetradecane caused loss of molecular species containing stearoyl groups. These results indicate that culturing the cells with 1-(carboxymethylthio)tetradecane causes looser packing and an increase in fluidity of the diacylphosphatidylethanolamine molecules in the membranes. 12-O-tetradecanoyl phorbol-13-acetate (TPA), platelet-derived growth factor (PDGF)-BB, or PDGF-AA stimulation of 1-(carboxymethylthio)tetradecane-treated cells resulted in decreased maximal levels of c-fos mRNA expression, indicating attenuation of signal transduction. Compared to cells not treated, the levels of both PDGF-alpha and PDGF-beta receptors were lower while GTPase- activating protein and phospholipase C-gamma levels were not altered in C3H/10T1/2Cl8 and C3H/10T1/2Cl16 cells cultured in the presence of 1-(carboxymethylthio)tetradecane. Our data demonstrate that 1-(carboxymethylthio)tetradecane-mediated changes in phospholipid structure and composition may affect PDGF- and TPA- mediated c-fos gene regulation in fibroblasts.[1]


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