Gene cluster for creatinine degradation in Arthrobacter sp. TE1826.
The genes encoding creatininase (CrnA; 258 residues) and creatinase (CreA; 411 residues) from Arthrobacter sp. TE1826 were cloned and sequenced. The genes form a cluster with the sarcosine oxidase gene (soxA) and its regulator gene (soxR), which were cloned previously. The deduced amino acid sequences of CrnA and CreA show 35.9% and 63.1% identity, respectively to the corresponding Pseudomonas enzymes. CrnA and CreA were purified from the recombinant strains and characterized. Other open reading frames ( creB and crnB), encoding proteins similar to several transporters, were found downstream of creA and crnA, respectively.[1]References
- Gene cluster for creatinine degradation in Arthrobacter sp. TE1826. Nishiya, Y., Toda, A., Imanaka, T. Mol. Gen. Genet. (1998) [Pubmed]
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