Disease relevance of Pyrene, 1,3-dinitro-

• Extracts from cells that had been grown without pyrene activated 1-nitropyrene, 1-amino-7-nitrofluorene, 2,7-dinitro-9-fluorenone, 1,3-dinitropyrene, 1,6-dinitropyrene, and 6-nitrochrysene to DNA-damaging products, as shown in Salmonella typhimurium tester strains by the reversion assay and by induction of the umuC gene [1].
• Sixty-minute incubation of 1,3-dinitropyrene (100 muM) or 1,4-dinitrophenol (100 muM) with a mixture of 150 muM NADH, 0.5 units of E. coli nitroreductase, 100 muM linoleic acid, 0.5 mM ferrous iron, and 100 muM 2'-deoxyadenosine (2'-dA) or 100 muM 2'-deoxyguanosine (2'-dG) were analyzed by liquid chromatography multistage mass spectrometry [2].

High impact information on Pyrene, 1,3-dinitro-

• Both rat cytochrome P-450 beta NF-B (P-450 IA1) and P-450ISF-G (P-450 IA2) inactivated 1,3-dinitropyrene, with the former being more effective [3].
• Out of the tested nitroarenes, 5 compounds, i.e., 1-nitropyrene, 1,3-dinitropyrene, 1,6-dinitropyrene, 1,8-dinitropyrene and 1-nitro-3-acetoxypyrene, clearly elicited positive responses of DNA repair [4].
• In vitro synthesis of 1,N(6)-etheno-2'-deoxyadenosine and 1,N(2)-etheno-2'-deoxyguanosine by 2,4-dinitrophenol and 1,3-dinitropyrene in presence of a bacterial nitroreductase [2].
• The results show that polycyclic nitroarenes differentially affect the test cell lines suggesting that some compounds, such as 1,3-dinitropyrene, are activated by cytochrome P4501A1, others, such as 1,6-dinitropyrene, by reductase(s) and acetyltransferase [5].
• The major mutagens in these materials were found to be 1-nitropyrene and 1,3-dinitropyrene on the basis of the result of gas chromatography/mass spectrometry and of fluorescence spectra of the samples [6].

References