Disease relevance of Tritosol

• The impregnation of tissue blocks with PPO (2,5-Diphenyloxazole) and POPOP (1,4-bis-2-(5-phenyloxazolyl)-benzene) during dehydration, infiltration and Epon embedding produced a 20% increase of reaction intensity in light microscope radioautographs using NTB2 emulsion and a 75% increase in electron microscope radioautographs using L4 emulsion [1].

High impact information on Tritosol

• The membranes were immersed in dimethyl ether, containing 10% of 2,5-diphenyloxazole prior to fluorography [2].
• A new method of preparative peptide mapping using 2,5-diphenyloxazole (PPO) fluor to detect peptides is described [3].
• In liver a single i.p. dose (500 mg/kg) enhanced cytochrome P-450 content 1.7-fold, ethoxycoumarin O-deethylase activity 2.5-fold, 2,5-diphenyloxazole hydroxylase activity 1.6-fold, epoxide hydrolase activity 2.5-fold, glutathione S-transferase activity 1.4-fold and UDPglucuronosyltransferase (4-methylumbelliferone) activity 2.3-fold in 3 days [4].
• The oxidative metabolism of 2,5-diphenyloxazole (PPO) is associated with 3-methylcholanthrene inducible cytochrome P-450 [5].

Anatomical context of Tritosol

• 2,5-Diphenyloxazole (PPO) is metabolized to one major fluorescent product by human liver and rat liver microsomes [6].

References