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MeSH Review

Peptide Mapping

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Disease relevance of Peptide Mapping


Psychiatry related information on Peptide Mapping


High impact information on Peptide Mapping

  • The 44 kd CD8-associated protein was identified as the heavy chain of the class I major histocompatibility antigen by depletion in preclearing experiments with anti-class I MHC antibody and by peptide mapping [8].
  • Peptide mapping of p34cdc2, p36CDC28, and human p34 revealed complete conservation of four tryptophan residues in the three proteins. p34 thus appears to be closely related to the two yeast proteins [9].
  • By peptide mapping, sequence, and immunological analyses, we show that lipocortin I and the 35 kd substrate for the EGF-receptor/kinase from A431 cells are the same protein [10].
  • The slower migrating micronuclear species, H3S, is indistinguishable from the macronuclear H3 by electrophoretic analyses in three gel systems and by partial proteolytic peptide mapping [11].
  • Peptide mapping has in all cases confirmed the identity of the products of cell-free synthesis with authentic alpha-tubulin, beta-tubulin and actin [12].

Chemical compound and disease context of Peptide Mapping


Biological context of Peptide Mapping


Anatomical context of Peptide Mapping

  • The variability of the MHC restricted receptor on murine T cells was examined by comparing tryptic peptide fingerprints of the receptor isolated fom three T cell hybridomas and a T cell tumor [23].
  • The 80 K phosphoproteins generated after activation of protein kinase C, both in cell-free conditions and in intact fibroblasts, are identical as judged by one and two-dimensional polyacrylamide slab gel electrophoresis and peptide mapping [24].
  • A 60,000-dalton protein fragment (Meyer, D. I., and B. Dobberstein, 1980, J. Cell Biol., 87:503-508) that had been shown previously to reconstitute the translocation activity of protease-digested membranes, was shown here by peptide mapping and immunological criteria to be derived from the SRP receptor [25].
  • This protein was identified by its mobility on one- and two-dimensional gel electrophoresis, by binding of antibodies to calf thymus HMG-1 to polypeptides electrophoretically separated and blotted on nitrocellulose paper, and by tryptic peptide mapping of radioiodinated polypeptides [26].
  • This yeast polypeptide is recognized by polyclonal antibodies against turkey p58, partitions entirely with the nuclear fraction, remains membrane bound after urea extraction of the nuclear envelopes, and is structurally similar to turkey p58 by peptide mapping criteria [27].

Associations of Peptide Mapping with chemical compounds


Gene context of Peptide Mapping

  • Flies also contain a cytoplasmic myosin heavy chain polypeptide that by immunological and peptide mapping criteria is clearly different from the major thoracic muscle isoform [33].
  • An in vitro kinase assay and subsequent tryptic peptide mapping revealed that Y1175 and Y1214 are the two major VEGF-A-dependent autophosphorylation sites [34].
  • These residues are also phosphorylated by purified ERK1 in vitro, as determined by a combination of phosphopeptide sequencing and 2-D peptide mapping [35].
  • Furthermore, anti-Ly-15.2 and anti-LFA-1 antibodies coprecipitate the same molecule from thymocyte lysates, and peptide mapping studies show that the Ly-15.2 and LFA-1 alpha chains are identical, as are the beta chains [36].
  • Photoaffinity labeling and peptide mapping studies indicate that the Ah receptor binds agonist at a domain that lies within this conserved N-terminal domain [37].

Analytical, diagnostic and therapeutic context of Peptide Mapping


  1. Sites of phosphorylation and mutation in regulatory subunit of cyclic AMP-dependent protein kinase from S49 mouse lymphoma cells: mapping to structural domains. Steinberg, R.A. J. Cell Biol. (1983) [Pubmed]
  2. Characterization of antigens recognized by monoclonal and polyclonal antibodies directed against uvomorulin. Peyriéras, N., Louvard, D., Jacob, F. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
  3. Absence of a structural basis for intracellular recognition and differential localization of nuclear and plasma membrane-associated forms of simian virus 40 large tumor antigen. Jarvis, D.L., Cole, C.N., Butel, J.S. Mol. Cell. Biol. (1986) [Pubmed]
  4. Specificity and Zn2+ enhancement of the S100B binding epitope TRTK-12. Barber, K.R., McClintock, K.A., Jamieson, G.A., Dimlich, R.V., Shaw, G.S. J. Biol. Chem. (1999) [Pubmed]
  5. ATP-dependent ferric hydroxamate transport system in Escherichia coli: periplasmic FhuD interacts with a periplasmic and with a transmembrane/cytoplasmic region of the integral membrane protein FhuB, as revealed by competitive peptide mapping. Mademidis, A., Killmann, H., Kraas, W., Flechsler, I., Jung, G., Braun, V. Mol. Microbiol. (1997) [Pubmed]
  6. Protein surface topology-probing by selective chemical modification and mass spectrometric peptide mapping. Suckau, D., Mak, M., Przybylski, M. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  7. Identification of heparin-binding domains in the amyloid precursor protein of Alzheimer's disease by deletion mutagenesis and peptide mapping. Clarris, H.J., Cappai, R., Heffernan, D., Beyreuther, K., Masters, C.L., Small, D.H. J. Neurochem. (1997) [Pubmed]
  8. Evidence for specific association between class I major histocompatibility antigens and the CD8 molecules of human suppressor/cytotoxic cells. Blue, M.L., Craig, K.A., Anderson, P., Branton, K.R., Schlossman, S.F. Cell (1988) [Pubmed]
  9. Identification of p34 and p13, human homologs of the cell cycle regulators of fission yeast encoded by cdc2+ and suc1+. Draetta, G., Brizuela, L., Potashkin, J., Beach, D. Cell (1987) [Pubmed]
  10. Two human 35 kd inhibitors of phospholipase A2 are related to substrates of pp60v-src and of the epidermal growth factor receptor/kinase. Huang, K.S., Wallner, B.P., Mattaliano, R.J., Tizard, R., Burne, C., Frey, A., Hession, C., McGray, P., Sinclair, L.K., Chow, E.P. Cell (1986) [Pubmed]
  11. Proteolytic processing of histone H3 in chromatin: a physiologically regulated event in Tetrahymena micronuclei. Allis, C.D., Bowen, J.K., Abraham, G.N., Glover, C.V., Gorovsky, M.A. Cell (1980) [Pubmed]
  12. Isolation of separate mRNAs for alpha- and beta-tubulin and characterization of the corresponding in vitro translation products. Cleveland, D.W., Kirschner, M.W., Cowan, N.J. Cell (1978) [Pubmed]
  13. Actin nascent chains are substrates for cyclic AMP-dependent phosphorylation in vivo. Steinberg, R.A. Proc. Natl. Acad. Sci. U.S.A. (1980) [Pubmed]
  14. Peptide mapping analysis of the avian progesterone receptor. Puri, R.K., Toft, D.O. J. Biol. Chem. (1986) [Pubmed]
  15. Evidence that there exist four classes of RNA tumor viruses which encode proteins with associated tyrosine protein kinase activities. Patschinsky, T., Sefton, B.M. J. Virol. (1981) [Pubmed]
  16. Single active-site histidine in D-xylose isomerase from Streptomyces violaceoruber. Identification by chemical derivatization and peptide mapping. Vangrysperre, W., Ampe, C., Kersters-Hilderson, H., Tempst, P. Biochem. J. (1989) [Pubmed]
  17. Human interleukin-5 expressed in Escherichia coli: assignment of the disulfide bridges of the purified unglycosylated protein. Proudfoot, A.E., Davies, J.G., Turcatti, G., Wingfield, P.T. FEBS Lett. (1991) [Pubmed]
  18. A novel integrin beta subunit is associated with the vitronectin receptor alpha subunit (alpha v) in a human osteosarcoma cell line and is a substrate for protein kinase C. Freed, E., Gailit, J., van der Geer, P., Ruoslahti, E., Hunter, T. EMBO J. (1989) [Pubmed]
  19. Differential expression and distribution of chicken skeletal- and smooth-muscle-type alpha-actinins during myogenesis in culture. Endo, T., Masaki, T. J. Cell Biol. (1984) [Pubmed]
  20. Distinct forms of both alpha and beta subunits are present in the human Ia molecular pool. Accolla, R.S., Gross, N., Carrel, S., Corte, G. Proc. Natl. Acad. Sci. U.S.A. (1981) [Pubmed]
  21. Systematic mapping of potential binding sites for Shc and Grb2 SH2 domains on insulin receptor substrate-1 and the receptors for insulin, epidermal growth factor, platelet-derived growth factor, and fibroblast growth factor. Ward, C.W., Gough, K.H., Rashke, M., Wan, S.S., Tribbick, G., Wang, J. J. Biol. Chem. (1996) [Pubmed]
  22. Human liver microsomal cytochrome P-450 mephenytoin 4-hydroxylase, a prototype of genetic polymorphism in oxidative drug metabolism. Purification and characterization of two similar forms involved in the reaction. Shimada, T., Misono, K.S., Guengerich, F.P. J. Biol. Chem. (1986) [Pubmed]
  23. The major histocompatibility complex-restricted antigen receptor on T cells in mouse and man: identification of constant and variable peptides. Kappler, J., Kubo, R., Haskins, K., Hannum, C., Marrack, P., Pigeon, M., McIntyre, B., Allison, J., Trowbridge, I. Cell (1983) [Pubmed]
  24. Phosphorylation of an acidic mol. wt. 80 000 cellular protein in a cell-free system and intact Swiss 3T3 cells: a specific marker of protein kinase C activity. Rodriguez-Pena, A., Rozengurt, E. EMBO J. (1986) [Pubmed]
  25. Protein translocation across the endoplasmic reticulum. II. Isolation and characterization of the signal recognition particle receptor. Gilmore, R., Walter, P., Blobel, G. J. Cell Biol. (1982) [Pubmed]
  26. High mobility group proteins of amphibian oocytes: a large storage pool of a soluble high mobility group-1-like protein and involvement in transcriptional events. Kleinschmidt, J.A., Scheer, U., Dabauvalle, M.C., Bustin, M., Franke, W.W. J. Cell Biol. (1983) [Pubmed]
  27. Lamin A, lamin B, and lamin B receptor analogues in yeast. Georgatos, S.D., Maroulakou, I., Blobel, G. J. Cell Biol. (1989) [Pubmed]
  28. Antibacterial activity of lysozyme and lactoferrin is inhibited by binding of advanced glycation-modified proteins to a conserved motif. Li, Y.M., Tan, A.X., Vlassara, H. Nat. Med. (1995) [Pubmed]
  29. Oxygen activation and reduction in respiration: involvement of redox-active tyrosine 244. Proshlyakov, D.A., Pressler, M.A., DeMaso, C., Leykam, J.F., DeWitt, D.L., Babcock, G.T. Science (2000) [Pubmed]
  30. Human homologue of murine T200 glycoprotein. Omary, M.B., Trowbridge, I.S., Battifora, H.A. J. Exp. Med. (1980) [Pubmed]
  31. Lung collagen heterogeneity. Synthesis of type I and type III collagen by rabbit and human lung cells in culture. Hance, A.J., Bradley, K., Crystal, R.G. J. Clin. Invest. (1976) [Pubmed]
  32. An osteopenic nonfracture syndrome with features of mild osteogenesis imperfecta associated with the substitution of a cysteine for glycine at triple helix position 43 in the pro alpha 1(I) chain of type I collagen. Shapiro, J.R., Stover, M.L., Burn, V.E., McKinstry, M.B., Burshell, A.L., Chipman, S.D., Rowe, D.W. J. Clin. Invest. (1992) [Pubmed]
  33. Identification of the gene for fly non-muscle myosin heavy chain: Drosophila myosin heavy chains are encoded by a gene family. Kiehart, D.P., Lutz, M.S., Chan, D., Ketchum, A.S., Laymon, R.A., Nguyen, B., Goldstein, L.S. EMBO J. (1989) [Pubmed]
  34. A single autophosphorylation site on KDR/Flk-1 is essential for VEGF-A-dependent activation of PLC-gamma and DNA synthesis in vascular endothelial cells. Takahashi, T., Yamaguchi, S., Chida, K., Shibuya, M. EMBO J. (2001) [Pubmed]
  35. ERK phosphorylation potentiates Elk-1-mediated ternary complex formation and transactivation. Gille, H., Kortenjann, M., Thomae, O., Moomaw, C., Slaughter, C., Cobb, M.H., Shaw, P.E. EMBO J. (1995) [Pubmed]
  36. The Ly-15 alloantigenic system: a genetically determined polymorphism of the murine lymphocyte function-associated antigen-1 molecule. Hogarth, P.M., Walker, I.D., McKenzie, I.F., Springer, T.A. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
  37. Cloning of the Ah-receptor cDNA reveals a distinctive ligand-activated transcription factor. Burbach, K.M., Poland, A., Bradfield, C.A. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  38. Continued expression of neonatal myosin heavy chain in adult dystrophic skeletal muscle. Bandman, E. Science (1985) [Pubmed]
  39. Cross-linking of Fas by antibodies to a peculiar domain of gp120 V3 loop can enhance T cell apoptosis in HIV-1-infected patients. Silvestris, F., Nagata, S., Cafforio, P., Silvestris, N., Dammacco, F. J. Exp. Med. (1996) [Pubmed]
  40. Rat hepatocytes in serum-free primary culture elaborate an extensive extracellular matrix containing fibrin and fibronectin. Stamatoglou, S.C., Hughes, R.C., Lindahl, U. J. Cell Biol. (1987) [Pubmed]
  41. Quantitation of two endogenous lactose-inhibitable lectins in embryonic and adult chicken tissues. Beyer, E.C., Barondes, S.H. J. Cell Biol. (1982) [Pubmed]
  42. Identification of two lysosomal membrane glycoproteins. Chen, J.W., Murphy, T.L., Willingham, M.C., Pastan, I., August, J.T. J. Cell Biol. (1985) [Pubmed]
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