Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Chemical Compound Review:

KST-1B4781 1-[(2R,4S,5R)-4-hydroxy-5...

Synonyms: AC1Q6GNV, AR-1B0775, LS-153783, AC1L2JN4, 04-Methyl-dT, ...

Dolan, M.E. et al., Montesano, R. et al., Ahmmed, Z. et al., Brennand, J. et al., Wani, A.A. et al., et al.

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of AIDS088695

The ability of O6-alkylguanine-DNA-alkyltransferases from Escherichia coli and from rat liver to repair O4-methylthymidine was determined using this methylated DNA as a substrate [1].

High impact information on AIDS088695

It was found that O4-methylthymidine represented only 0.06 +/- 0.02% of the total methylation and that the ratio of O6-methyldeoxyguanosine:O4-methylthymidine was 126 +/- 31 [1].
This shows that the rat O6-alkylguanine-DNA-alkyltransferase is not able to protect against the possible hazards of the promutagenic lesion, O4-methylthymidine, but the very low extent of formation of this product may limit its significance in carcinogenesis and mutagenesis [1].
Calf thymus DNA was methylated by reaction with N-[3H]-methyl-N-nitrosourea and the content of O6-methyldeoxyguanosine, 3-methylthymidine and O4-methylthymidine was determined [1].
High concentrations of O2-methylthymidine, 3-methylthymidine and O4-methylthymidine (O4-MedT) have little effect on the colony forming ability of cultured Chinese hamster (V79) cells [2].
The suitability of various substrates was established in assays using E. coli extracts that removed O4-methylthymidine (O4-MedT), O2-MedT, and O6-methylguanine (O6-MeG) [3].

Biological context of AIDS088695

Alkylation of poly(dT) by N-[methyl-3H] (N-nitrosomethylurea) and subsequent annealing with poly(dA) yield a substrate containing O2 and O4-methylthymidine, 3-methylthymidine and phosphotriesters [4].

Associations of AIDS088695 with other chemical compounds

Various methods for detecting DNA alkylation adducts are described briefly, with emphasis on immunoassays using antibodies against O6-methyldeoxyguanosine (O6-medGua), O4-methylthymidine (O4-meThy) and 7-methyldeoxyguanosine (7-medGua) [5].

Analytical, diagnostic and therapeutic context of AIDS088695

The incorporation of O4-methylthymidine into V79A cell DNA when present in the cell culture medium [6].

References

Extent of formation of O4-methylthymidine in calf thymus DNA methylated by N-methyl-N-nitrosourea and lack of repair of this product by rat liver O6-alkylguanine-DNA-alkyltransferase. Dolan, M.E., Pegg, A.E. Carcinogenesis (1985) [Pubmed]
The effects of methylated thymidines upon cultures of V79 cells and the mechanism of incorporation of O4-methylthymidine into their DNA. Brennand, J., Saffhill, R., Fox, M. Carcinogenesis (1982) [Pubmed]
Repair of DNA O-alkylation damage by various human organs. Wani, A.A., Wani, G., D'Ambrosio, S.M. Biochem. Biophys. Res. Commun. (1985) [Pubmed]
Enzymatic repair of O-alkylated thymidine residues in DNA: involvement of a O4-methylthymine-DNA methyltransferase and a O2-methylthymine DNA glycosylase. Ahmmed, Z., Laval, J. Biochem. Biophys. Res. Commun. (1984) [Pubmed]
Detection in human cells of alkylated macromolecules attributable to exposure to nitrosamines. Montesano, R., Brésil, H., Degan, P., Martel-Planche, G., Serres, M., Wild, C.P. IARC Sci. Publ. (1988) [Pubmed]
The incorporation of O4-methylthymidine into V79A cell DNA when present in the cell culture medium. Saffhill, R., Fox, M. Carcinogenesis (1980) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.