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MeSH Review


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Disease relevance of Alkylation


High impact information on Alkylation


Chemical compound and disease context of Alkylation


Biological context of Alkylation


Anatomical context of Alkylation


Associations of Alkylation with chemical compounds


Gene context of Alkylation

  • In these, but not in NR1-NR2A, the DTT effect was rendered insensitive to reoxidation by alkylation [29].
  • MGT1 transcript levels are not increased in response to DNA alkylation damage, nor is the MGT1 MTase involved in the regulation of the yeast 3-methyladenine DNA glycosylase gene (MAG) [30].
  • The NH(2)- and COOH-terminal Stat domains are generally dispensable, whereas alkylation of a single cysteine residue blocked cytokine-independent nuclear translocation and thus implicated the linker domain into the cycling of Stat1 [31].
  • This was done with iodide (anion binding) and bromopyruvic acid (alkylation), and the respective assays evaluated as diagnostic tools for CA II deficiency in human red cells [32].
  • The apparent Km values for addition of GalNAc to oligosaccharides on hCG alpha and hCG beta, 13.0 and 6.2 microM, respectively, are not altered by reduction and alkylation [33].

Analytical, diagnostic and therapeutic context of Alkylation

  • Human O(6)-alkylguanine-DNA alkyltransferase (AGT), which directly reverses endogenous alkylation at the O(6)-position of guanine, confers resistance to alkylation chemotherapies and is therefore an active anticancer drug target [34].
  • Upon reduction and alkylation, the antigen-binding GIF dissociated into a 62- to 64-kDa protein which gave positive ELISA with antibody 110BH3 but no signal with antibody 388F1, and a 15-kDa protein, which gave ELISA signal with the 388F1 but not with 110BH3 [35].
  • DNA alkylation was essential for the formation of top1-mediated cleavage complexes by Et743, and the distribution of the drug-induced top1 sites was different for Et743 and camptothecin. top1-DNA complexes were also detected in Et743-treated CEM cells by using cesium chloride gradient centrifugation followed by top1 immunoblotting [36].
  • Flow cytometry indicated that in vivo alkylation resistance in Aag null BM cells may be restricted to cells of the myeloid lineage [37].
  • Circular dichroism studies of the ethylene adduct and of the ring D regioisomer of N-ethylprotoporphyrin IX obtained by alkylation of the prosthetic heme of hemoglobin have been used to determine which face of cytochrome P-450 heme is alkylated by the unsaturated substrates [38].


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