High impact information on N-phenylcarbamic acid ethyl ester

• Activation of carzelesin requires two steps, (a) hydrolysis of a phenylurethane substituent to form U-76073, followed by (b) ring closure to form the cyclopropyl-containing DNA-reactive U-76074 [1].
• Auxin-induced tyrosinylated alpha-tubulin, correlated with an elevated sensitivity of growth to antimicrotubular compounds such as ethyl-N-phenylcarbamate (EPC) [2].
• We determined the affinity of alpha-tubulin to EPC and found that it was dramatically increased when the tubulin was de-tyrosinylated [2].
• Channels at the catalytic site of glycogen phosphorylase b: binding and kinetic studies with the beta-glycosidase inhibitor D-gluconohydroximo-1,5-lactone N-phenylurethane [3].
• Selective inhibition of transition from sexual agglutination to zygote formation by ethyl N-phenylcarbamate in Saccharomyces cerevisiae [4].

Biological context of N-phenylcarbamic acid ethyl ester

• When diploid cells of Saccharomyces cerevisiae were incubated in acetate growth media containing 2.5 mM ethyl N-phenylcarbamate (EPC), bud initiation was inhibited preferentially, and eventually overgrown, unbudded cells accumulated [5].
• The effect of taxol and ethyl-N-phenylcarbamate (EPC) on the growth and gravitropism of maize roots and coleoptiles was studied [6].
• Phenylurethane type : Incomplete and reversible action, which leads to easy production of pluripolar mitoses; 5 [7].

Analytical, diagnostic and therapeutic context of N-phenylcarbamic acid ethyl ester

• The method involves affinity chromatography with ethyl N-phenylcarbamate and Sepharose [8].

References