Disease relevance of scrA

• The predicted amino acid sequences of the two open reading frames exhibited approximately 45 and 70% identity with those encoded by scrA and scrB, respectively, from Streptococcus mutans GS5 [1].
• Sequence analysis of scrA and scrB from Streptococcus sobrinus 6715 [1].
• The cloned scrA gene of S. sobrinus 6715-10 coding for the EIISuc of the sucrose-specific phosphoenolpyruvate phosphotransferase system was interrupted by the insertion of a streptococcal spectinomycin resistance gene active in E. coli and streptococci [2].
• The nucleotide sequence of the Vibrio alginolyticus sucrose uptake-encoding region was determined, and contained two genes, scrA and scrK [3].

High impact information on scrA

• It was previously shown that expression of both scrA and scrB is similarly induced by sucrose [4].
• The results showed that ScrR bound specifically to the promoter regions of both scrA and scrB [4].
• The scrA gene encodes an enzyme IISucrose (EIIScr) protein of the phosphoenolpyruvate dependent phosphotransferase system and the scrK gene encodes a fructokinase [3].
• The nucleotide sequence of the scrA region revealed that this gene was located immediately upstream from the scrB gene and divergently transcribed from the opposite DNA strand [5].
• The cloned scrA gene product was identified as a 68-kilodalton protein by minicell analysis after isolation of the gene in plasmid pD4 [5].

Chemical compound and disease context of scrA

• The complete nucleotide sequences of Streptococcus sobrinus 6715 scrA and scrB, which encode sucrose-specific enzyme II of the phosphoenolpyruvate-dependent phosphotransferase system and sucrose-6-phosphate hydrolase, respectively, have been determined [1].

Biological context of scrA

• The Streptococcus mutans GS-5 scrA gene coding for enzyme IIScr of the phosphoenolpyruvate-dependent sucrose phosphotransferase system (PTS) was localized upstream from the scrB gene coding for sucrose-6-phosphate hydrolase activity after Mu dE transposon mutagenesis of plasmid pMH613 [5].
• Three strains of S. mutans harboring reporter gene fusions to the gene promoter regions of the gtfBC genes, ftf, and scrA were grown in a Rototorque biofilm fermenter in a tryptone-yeast extract-sucrose medium [6].

References