The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

pcaR  -  pca regulon regulatory protein

Pseudomonas putida KT2440

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of pcaR


High impact information on pcaR

  • DNA sequencing, mutational analysis, and complementation studies revealed that pcaR encodes a regulatory protein required for induction of at least four of the enzymes of the beta-ketoadipate pathway and that pcaF encodes beta-ketoadipyl-coenzyme A thiolase, the last enzyme in the pathway [2].
  • Insertional inactivation mutagenesis, using Tn5 and mini-Tn5 transposons, was used to locate, clone, and sequence this pcaR regulatory gene [1].
  • Fusion analyses showed that pcaI and pcaJ constitute an operon which requires beta-ketoadipate or its nonmetabolizable analog, adipate, as well as the pcaR regulatory gene for induction [3].
  • An additional P. putida gene, pcaR, was shown to be required for expression of the pca structural genes in response to beta-ketoadipate [4].
  • The regulatory pcaR gene is located about 15 kbp upstream from the pcaBDC operon [4].

Biological context of pcaR

  • Comparisons of the pcaIJ and pcaR promoter regions revealed conservation of a 15-bp sequence centered around the -10 region in both sequences [1].


  1. Characterization of the pcaR regulatory gene from Pseudomonas putida, which is required for the complete degradation of p-hydroxybenzoate. Romero-Steiner, S., Parales, R.E., Harwood, C.S., Houghton, J.E. J. Bacteriol. (1994) [Pubmed]
  2. Identification of the pcaRKF gene cluster from Pseudomonas putida: involvement in chemotaxis, biodegradation, and transport of 4-hydroxybenzoate. Harwood, C.S., Nichols, N.N., Kim, M.K., Ditty, J.L., Parales, R.E. J. Bacteriol. (1994) [Pubmed]
  3. Regulation of the pcaIJ genes for aromatic acid degradation in Pseudomonas putida. Parales, R.E., Harwood, C.S. J. Bacteriol. (1993) [Pubmed]
  4. Cloning and expression of pca genes from Pseudomonas putida in Escherichia coli. Hughes, E.J., Shapiro, M.K., Houghton, J.E., Ornston, L.N. J. Gen. Microbiol. (1988) [Pubmed]
WikiGenes - Universities