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Gene Review:

susC - SusC, outer membrane protein involved in...

Bacteroides thetaiotaomicron VPI-5482

Reeves, A.R. et al., Cho, K.H. et al., Wei, B. et al., Reeves, A.R. et al., Shipman, J.A. et al.

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Disease relevance of susC

The closest homologues of OmpW included the outer membrane proteins SusC of Bacteroides thetaiotaomicron and RagA of Porphyromonas gingivalis [1].

High impact information on susC

Consistent with this, nondenaturing gel analysis revealed that in a strain producing SusC, SusD, and SusE, the SusCD complex was partially destabilized [2].
In this paper, we report that four other outer membrane proteins, SusC through SusF, are responsible for starch binding [3].
SusC protein proved to be essential for utilization not only of starch but also of intermediate-sized maltooligosaccharides (maltose to maltoheptaose) [4].
The amino acid sequence, together with results of gene disruption experiments, suggested that SusC was not an amylolytic enzyme [5].
SusC had a possible signal sequence of between 20 and 39 amino acids, depending on which N-terminal methionine initiates the start of the protein [5].

Associations of susC with chemical compounds

Both formaldehyde cross-linking and nondenaturing gel electrophoresis experiments showed that SusC and SusD interact to form a complex [2].

Other interactions of susC

Two of these, SusC and SusD, have been shown by genetic analysis to be required for starch binding [2].

Analytical, diagnostic and therapeutic context of susC

Antibodies were raised against SusC, and the outer membrane location of SusC could be confirmed by Western blot (immunoblot) analysis [5].

References

Molecular cloning of a Bacteroides caccae TonB-linked outer membrane protein identified by an inflammatory bowel disease marker antibody. Wei, B., Dalwadi, H., Gordon, L.K., Landers, C., Bruckner, D., Targan, S.R., Braun, J. Infect. Immun. (2001) [Pubmed]
Biochemical analysis of interactions between outer membrane proteins that contribute to starch utilization by Bacteroides thetaiotaomicron. Cho, K.H., Salyers, A.A. J. Bacteriol. (2001) [Pubmed]
Characterization of four outer membrane proteins involved in binding starch to the cell surface of Bacteroides thetaiotaomicron. Shipman, J.A., Berleman, J.E., Salyers, A.A. J. Bacteriol. (2000) [Pubmed]
Characterization of four outer membrane proteins that play a role in utilization of starch by Bacteroides thetaiotaomicron. Reeves, A.R., Wang, G.R., Salyers, A.A. J. Bacteriol. (1997) [Pubmed]
A Bacteroides thetaiotaomicron outer membrane protein that is essential for utilization of maltooligosaccharides and starch. Reeves, A.R., D'Elia, J.N., Frias, J., Salyers, A.A. J. Bacteriol. (1996) [Pubmed]

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