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Gene Review:

EDDM3A - epididymal protein 3A

Homo sapiens

Synonyms: EP3A, Epididymal secretory protein E3-alpha, FAM12A, HE3-ALPHA, HE3-alpha, ...

Regan, J.W. et al., Burkey, T.H. et al., Kirchhoff, C. et al.

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High impact information on FAM12A

They also differ in content with the EP3A containing 9 serine and threonines in its carboxyl terminus and the EP3E none [1].
2. Three of these variants, i.e. the EP3A, EP3E and EP3D, share the same primary amino acid sequence except for their carboxyl termini, which diverge from one another at the same point, approximately 10 amino acids away from the end of the seventh membrane spanning domain of the receptor [1].
Using a reporter gene assay, as a measure of intracellular cyclic AMP levels, the EP3A coupled more efficiently to the inhibition of adenylyl cyclase than did the EP3E [1].
In crude lysates of COS-7 cells transfected with the human EP3A, 1 microM PGE2 stimulated MAP kinase activity approximately 1.3-fold with an EC50 of approximately 6 nM [2].
More detailed sequence and PCR analysis identified two different but homologous gene transcripts, HE3 alpha and HE3 beta [3].

Anatomical context of FAM12A

RNase protection assays showed that both the HE3 alpha and HE3 beta, but not the HE3 gamma genes, are expressed in the human epididymis [3].

Associations of FAM12A with chemical compounds

This activation of MAP kinase activity by the EP3A receptor may explain the proliferative actions of PGE2 in some tissues [2].

References

Molecular cloning and expression of human EP3 receptors: evidence of three variants with differing carboxyl termini. Regan, J.W., Bailey, T.J., Donello, J.E., Pierce, K.L., Pepperl, D.J., Zhang, D., Kedzie, K.M., Fairbairn, C.E., Bogardus, A.M., Woodward, D.F. Br. J. Pharmacol. (1994) [Pubmed]
Activation of mitogen-activated protein kinase by the human prostaglandin EP3A receptor. Burkey, T.H., Regan, J.W. Biochem. Biophys. Res. Commun. (1995) [Pubmed]
Major human epididymis-specific gene product, HE3, is the first representative of a novel gene family. Kirchhoff, C., Pera, I., Rust, W., Ivell, R. Mol. Reprod. Dev. (1994) [Pubmed]

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