Disease relevance of SCO2210

• Investigation of the functional properties and regulation of three glutamine synthetase-like genes in Streptomyces coelicolor A3(2) [1].
• In heterologous expression experiments, glnA2, glnA3, and glnA4, in contrast to glnA and glnII, were not capable of complementing the L: -glutamine auxotrophy of an Escherichia coli glnA mutant [1].

High impact information on SCO2210

• Recombinant Ptr interacted with the upstream region of ptr, but not with the promoter region of glnII [2].
• Besides glnII, this region contains four ORFs (orf1-orf4) [2].
• While homologues of orf1 and orf2 were also found in the glnII region of the S. viridochromogenes chromosome, this was not the case for orf3 and orf4, which encode a putative hydrolase and a transcriptional regulator (Ptr) of the MarR family, respectively [2].
• The DNA region showed 90% nucleotide identity with the Streptomyces coelicolor A3(2) glnA gene, but no significant nucleotide sequence similarity with the glnII (GSII) gene of S. viridochromogenes [3].
• Neither glnA nor glnII single mutants required glutamine for growth and both were normal in their sporulation [3].

Analytical, diagnostic and therapeutic context of SCO2210

• The involvement of ptr in glnII regulation was studied by gel retardation assays [2].
• The chromosomal glnA and glnII genes of S. viridochromogenes were disrupted by site-specific mutagenesis [3].

References