High impact information on C14orf172

• The essential Gcd10p-Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA [1].
• In fact, the lethality of a GCD14 deletion was suppressed by hcIMT4, indicating that the essential function of Gcd14p is required for biogenesis of tRNAiMet [2].
• Gcd10p and Gcd14p were first identified genetically as repressors of GCN4 mRNA translation in Saccharomyces cerevisiae [2].
• Here we show that Gcd14p is an essential protein with predicted binding motifs for S-adenosylmethionine, consistent with a direct function in tRNA methylation [2].
• Our findings suggest that Gcd14p and Lhp1p cooperate in the maturation of a subset of RNA polymerase III transcripts [2].

Biological context of C14orf172

• It is predicted that the prokaryotic proteins are genuine tRNA:m(1)A MTases based on similarity of their predicted active site to the Gcd14p family [3].
• The results suggest that the 'catalytic' Gcd14p and 'substrate binding' Gcd10p are related to each other and to a group of prokaryotic open reading frames, which were previously annotated as hypothetical protein isoaspartate MTases in sequence databases [3].

References