Disease relevance of yscM1

• The expression of yop genes is regulated in response to environmental cues, which results in a cascade of type III secretion reactions. yscM1 and yscM2 negatively regulate the expression of Yersinia enterocolitica yop genes [1].

High impact information on yscM1

• Overexpression of this gene, called yscM2, like overexpression of IcrQ and yscM1, blocked Yop secretion [2].
• Overexpression of yscM1 drastically reduced the expression of a yopH-cat reporter gene when tested in a pYV+ background [2].
• A double yscM1, yscM2 mutant had the same phenotype as that of the IcrQ mutant [2].
• The 8.5 kb virC region, involved in the specific transport of the Yops, is a single operon containing 13 open reading frames called yscA to yscM [3].
• The sequence 5'-AUAAA-3' is located in the 5' yop UTRs, and mutations that alter the sequence motif either reduced or abolished yscM1- and yscM2-mediated regulation [1].

Biological context of yscM1

• We analyzed the 8.5-kb virC region and showed that it is most probably a single operon containing 13 open reading frames called yscA to yscM (for Yop secretion) [4].
• The yscM gene shares homology with yopH, the adjacent gene on the pYV plasmid [4].
• Yersinia enterocolitica type III secretion: yscM1 and yscM2 regulate yop gene expression by a posttranscriptional mechanism that targets the 5' untranslated region of yop mRNA [1].

References