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Gene Review:

fimY - fimbrial regulatory protein FimY

Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Yeh, K.S. et al., Tinker, J.K. et al., Tinker, J.K. et al.

Yeh, Chen, Liao, Chang, Lo,

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Disease relevance of fimY

In addition, transcription from the cloned fimY promoter was not detected in Escherichia coli unless both FimZ and FimY were present, indicating that these proteins also act as coactivators of fimY expression [1].
Therefore, fimY is a promising target gene to detect the presence of Salmonella species [2].

High impact information on fimY

In addition, this mutant could be complemented by transformation with fimY altered in the first three rare arginine codons [3].
FimY production in this mutant could be restored to high levels when fimU was introduced on a plasmid, and also when three rare arginine codons, located within the first 14 positions within fimY, were exchanged for major arginine codons [3].
Extensive in vitro DNA binding assays using extracts from E. coli that overexpress FimY, as well as partially purified FimY, were unable to identify a specific interaction between FimY and the fimA or fimY promoter [1].
Herein, two primers internal to the fimY gene of S. typhimurium are used to investigate the distribution of the fimY homologous sequence among 45 Salmonella serovars and 20 non-Salmonella species by using PCR [2].
Although fimY gene of Salmonella typhimurium is involved in regulating type 1 fimbrial expression, the amino acid sequence of FimY shares very little homology with other known prokaryotic proteins in the GenBank database [2].

Biological context of fimY

Experimental results indicated that only Salmonella species possessed the fimY homologous sequence, subsequently generating the specific 526-bp DNA fragments [2].
This study evaluated the suitability of fimY gene amplification by PCR as an effective means of detecting Salmonella species [2].

Analytical, diagnostic and therapeutic context of fimY

Reverse transcriptase-polymerase chain reaction confirmed that the fimY transcript was present at similar levels in the fimU mutant and parental strain [3].
A combining of pre-enrichment step in buffered peptone water and PCR amplification of fimY allowed the detection of S. typhimurium at the concentration of 3.4 x 10(0) CFU/ml from the swab sample [2].

References

Characterization of FimY as a coactivator of type 1 fimbrial expression in Salmonella enterica serovar Typhimurium. Tinker, J.K., Clegg, S. Infect. Immun. (2000) [Pubmed]
PCR amplification of the Salmonella typhimurium fimY gene sequence to detect the Salmonella species. Yeh, K.S., Chen, T.H., Liao, C.W., Chang, C.S., Lo, H.C. Int. J. Food Microbiol. (2002) [Pubmed]
Control of FimY translation and type 1 fimbrial production by the arginine tRNA encoded by fimU in Salmonella enterica serovar Typhimurium. Tinker, J.K., Clegg, S. Mol. Microbiol. (2001) [Pubmed]

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