Disease relevance of fimA

• Extensive in vitro DNA binding assays using extracts from E. coli that overexpress FimY, as well as partially purified FimY, were unable to identify a specific interaction between FimY and the fimA or fimY promoter [1].
• Identification of ancillary fim genes affecting fimA expression in Salmonella typhimurium [2].

High impact information on fimA

• Studies using the fimY-lacZ construct reveal that expression of this gene varies with environmental conditions in a manner similar to fimA expression [1].
• A single gene, fimA, encodes the major fimbrial unit [3].
• The goal of this study was to evaluate the suitability of the fimA gene amplification by PCR as a specific method for detection of Salmonella strains [3].
• The fimbrin-encoding genes were not encoded by the virulence plasmids, but were located on chromosomal DNA fragments [4].
• Bacterial lysates, derived from strains transformed with recombinant plasmids possessing a fimZ gene, demonstrated DNA binding activity with a fragment containing the fimA promoter [5].

Biological context of fimA

• These data are consistent with FimZ being a transcriptional activator of fimA, and FimZ acts by binding to the promoter region [5].
• 2. The overall amino acid composition and the N-terminal amino acid sequence of the SEF 21 fimbrin were distinct from those of SEF 14 but were virtually identical to the predicted sequence for type 1 fimbrin of Salmonella typhimurium [6].
• Predominantly mature fimbrin (14,000 Mr) was produced by clones containing the 5.3-kb insert, whereas the original cosmid clone predominantly accumulated a prefimbrin precursor (16,500 Mr) [7].
• Mini-Mu and TnphoA (Tn5 IS50L::phoA) transposon mutagenesis was used to identify a 2- to 3.5-kb region downstream of fimA that affected fimbrin production and processing [7].
• Downstream of fimA, an open reading frame was found, named fimI, able to encode a fimbrin-like protein [8].

Associations of fimA with chemical compounds

• This fimbrin contained 40% hydrophobic amino acids and lacked cysteine [9].

Other interactions of fimA

• Regulation of the gene, fimA, encoding the major fimbrial subunit of S. typhimurium S6704 was examined by using a lambda fimA-lacZ lysogen [2].

Analytical, diagnostic and therapeutic context of fimA

• Cultures were screened by inoculating a single colony of bacteria directly into a PCR mixture containing a pair of primers specific for the fimA gene [3].

References