High impact information on galK

• In a galactokinase mutant, however, the galactose receptor is inducible by galactose [1].
• To study the regulation of orfF expression directly, transcriptional and translational fusions of orfF to galK and lacZ, respectively, were constructed and the level of expression of the reporter genes was determined under different growth conditions [2].
• Additional evidence for translational control was provided by the finding that synthesis of galactokinase mediated from a pyrC-galK transcriptional fusion is not regulated by pyrimidines [3].
• The nature of the dhuA and argTr promoters of the operons coding for these permeases was analyzed by placing the galactokinase gene under their control (in vector pKO-1). argTr was found to respond to nitrogen regulation [4].
• Suppression of dnaA mutation by F'lac and cryptic LT2 plasmid was used for selecting clones containing these plasmids integrated into rfa and galK genes [5].

Biological context of galK

• Subcloning of a 184-base-pair DNA fragment, which included 118 base pairs upstream from the transcriptional start, and the first eight codons of the pyrC structural gene, into a galK expression vector, established that the pyrC promoter and regulatory region are harbored on this fragment [6].

References