Disease relevance of purF

• Synthesis of thiamine in Salmonella typhimurium independent of the purF function [1].

High impact information on purF

• Mutations in this gene, which result in an m(1)G37 deficiency in the tRNA, in a purF mutant mediate PurF-independent thiamine synthesis [2].
• In Salmonella enterica serovar Typhimurium a mutation in the purF gene encoding the first enzyme in the purine pathway blocks, besides the synthesis of purine, the synthesis of thiamine when glucose is used as the carbon source [2].
• On carbon sources other than glucose, a purF mutant does not require thiamine, since the alternative pyrimidine biosynthetic (APB) pathway is activated [2].
• Gas chromatography-mass spectrometry analyses indicated that both of these compounds were incorporated into the pyrimidine moiety of TPP in a purF mutant [3].
• Complementation analysis demonstrated that the HMP requirement of the purF rseB strain was due to polarity of the insertion in rseB on the downstream rseC gene [4].

Biological context of purF

• The orientation of this operon relative to purF has been established by three-point crosses as being: purF duhA hisJ hisP [5].
• An insertion in rseB, the third gene in the rpoE rseABC gene cluster at 57 min, prevented HMP synthesis in a purF mutant [4].

Associations of purF with chemical compounds

• The alternative route of B1 synthesis appears to require enzymes which act subsequent to the purF enzyme in the purine pathway [1].
• The alternative purF function is shown here to be affected by temperature and exogenous pantothenate [6].
• We established that in both bacterial species these genes were cotransducible with the neighboring histidine transport operon and were distally located relative to purF. pta mutants were sensitive to the dye alizarin yellow and were unable to grow on medium containing inositol as a carbon source [7].

References