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Gene Review:

dsbA - thiol:disulfide interchange protein DsbA

Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Gallant, C.V. et al., Rodríguez-Peña, J.M. et al., Kang, J.W. et al., Ellermeier, C.D. et al., Turcot, I. et al., et al.

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Disease relevance of dsbA

H-NS represses Salmonella enterica serovar Typhimurium dsbA expression during exponential growth [1].
The dlp gene was able to complement the DTT-sensitive phenotype, the inability to metabolize glucose 1-phosphate and the low alkaline phosphatase activity of a dsbA mutant of Escherichia coli [2].

High impact information on dsbA

Here we show that the hilA regulators RtsA, HilD, and HilC can each induce transcription of dsbA, which encodes a periplasmic disulfide bond isomerase [3].
Direct binding of H-NS to the dsbA promoter region was verified using purified H-NS in electrophoretic mobility shift assays [1].
Strains with mutations created by transposon mutagenesis were screened for strains with altered expression of dsbA [1].
These null alleles were constructed by partial deletion of the dsbA-coding region and then insertion of an antibiotic resistance marker in the gene [4].
Based on amino acid sequence homology, ORF1 and ORF2 are designated as astA and dsbA, respectively [5].

Biological context of dsbA

Sequencing revealed two contiguous open reading frames (ORF1 and ORF2) on the same strand and based on amino acid sequence homology, they were designated as astA and dsbA, respectively [6].

Analytical, diagnostic and therapeutic context of dsbA

Northern blot analysis with a dsbA-specific probe showed an increase in dsbA transcript during exponential phase of growth [1].

References

H-NS represses Salmonella enterica serovar Typhimurium dsbA expression during exponential growth. Gallant, C.V., Ponnampalam, T., Spencer, H., Hinton, J.C., Martin, N.L. J. Bacteriol. (2004) [Pubmed]
Homologous regions of the Salmonella enteritidis virulence plasmid and the chromosome of Salmonella typhi encode thiol: disulphide oxidoreductases belonging to the DsbA thioredoxin family. Rodríguez-Peña, J.M., Alvarez, I., Ibáñez, M., Rotger, R. Microbiology (Reading, Engl.) (1997) [Pubmed]
RtsA coordinately regulates DsbA and the Salmonella pathogenicity island 1 type III secretion system. Ellermeier, C.D., Slauch, J.M. J. Bacteriol. (2004) [Pubmed]
Isolation and characterization of a chromosomally encoded disulphide oxidoreductase from Salmonella enterica serovar Typhimurium. Turcot, I., Ponnampalam, T.V., Bouwman, C.W., Martin, N.L. Can. J. Microbiol. (2001) [Pubmed]
Cloning and sequencing of the astA gene encoding arylsulfate sulfotransferase from Salmonella typhimurium. Kang, J.W., Kwon, A.R., Kim, D.H., Choi, E.C. Biol. Pharm. Bull. (2001) [Pubmed]
Cloning, sequence analysis, and characterization of the astA gene encoding an arylsulfate sulfotransferase from Citrobacter freundii. Kang, J.W., Jeong, Y.J., Kwon, A.R., Yun, H.J., Kim, D.H., Choi, E.C. Arch. Pharm. Res. (2001) [Pubmed]

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