Disease relevance of rpoB

• The mutations were mapped at min 89 of the Salmonella chromosome, near rpoB, by transduction [1].
• Suppression of the DnaA phenotype by mutations in the rpoB cistron of ribonucleic acid polymerase in Salmonella typhimurium and Escherichia coli [2].

High impact information on rpoB

• Oral vaccination of pigs with an invasive gyrA-cpxA-rpoB Salmonella Typhimurium mutant [3].
• These two mutants, which are only marginally affected in their response to Nov, are normally sensitive to Rif and thus might be representative of a new class of rpoB alleles [4].
• In a subsequent experiment, we found that mutants selected for low-level resistance to Nal include isolates harboring mutations genetically linked to the rpoB locus; of two such mutants studied, one is temperature-sensitive for growth [4].
• The Rif-resistant and Rif-sensitive rpoB alleles that increase resistance to gyrase inhibitors have one property in common: they all suppress, to varying degrees, the defect in his operon regulation (transcriptional deattenuation) caused by a gyrase defect or inhibition by novobiocin [4].

Biological context of rpoB

• It is concluded, therefore, that certain rpoB mutations may suppress the DnaA phenotype [2].

References