The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Chemical Compound Review

Oprea1_555120     [5-hydroxy-6-[2-hydroxy-3- [[4-hydroxy-3-(3...

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of novobiocin


High impact information on novobiocin


Chemical compound and disease context of novobiocin


Biological context of novobiocin


Anatomical context of novobiocin


Associations of novobiocin with other chemical compounds


Gene context of novobiocin

  • Evidence that this promoter coupling is DNA supercoiling-dependent is provided by the observation that a novobiocin-induced decrease in global negative superhelicity results in an increase in ilvY promoter activity and a decrease in ilvC promoter activity predicted by the in vitro data [25].
  • Treatment of cells with either geldanamycin or novobiocin, two pharmacological inhibitors of Hsp90 causes the destabilization of LKB1 [26].
  • Expression and analysis of two gyrB genes from the novobiocin producer, Streptomyces sphaeroides [27].
  • The mukB mutant was oversensitive to novobiocin and this susceptibility was suppressed in the mukBseqA strain, suggesting possible roles of MukB and SeqA in maintaining chromosome topology [28].
  • Transcription from the fimA promoter was unaffected by changes in DNA supercoiling whether caused by the introduction of a topA::Tn10 mutation or by inhibition of DNA gyrase with the antibiotic novobiocin [29].

Analytical, diagnostic and therapeutic context of novobiocin

  • Partial purification of DNA gyrase by affinity chromatography revealed the presence of two such activities (differing in their responses to novobiocin) in the clone [4].
  • The acquisition of maturation markers and changes in the activity, level, and phosphorylation of Topo II were determined after treatment with either novobiocin, a Topo II inhibitor, or PMA [30].
  • Plasma novobiocin levels > or = 100 micrograms/mL, which are associated with reversal of drug resistance in animal models, were consistently seen at dose levels greater than 2 g [9].
  • It has also been suggested that novobiocin-induced inhibition of repair is mediated by alterations of chromatin structure recognizable by electron microscopy as gross chromatin clumping [31].
  • The water-mediated hydrogen bonds linking novobiocin to the mutant protein make a favorable enthalpic contribution, whereas the immobilization of the water leads to an entropic cost and a reduction in the heat capacity of the system [32].


  1. Stimulation of a Chlamydomonas chloroplast promoter by novobiocin in situ and in E. coli implies regulation by torsional stress in the chloroplast DNA. Thompson, R.J., Mosig, G. Cell (1987) [Pubmed]
  2. Effect of novobiocin on initiation of DNA replication in Bacillus subtilis. Ogasawara, N., Seiki, M., Yoshikawa, H. Nature (1979) [Pubmed]
  3. Novobiocin inhibition of simian virus 40 DNA replication. Edenberg, H.J. Nature (1980) [Pubmed]
  4. Cloning and characterization of a DNA gyrase B gene from Streptomyces sphaeroides that confers resistance to novobiocin. Thiara, A.S., Cundliffe, E. EMBO J. (1988) [Pubmed]
  5. A mutant of Mycobacterium smegmatis defective in the biosynthesis of mycolic acids accumulates meromycolates. Liu, J., Nikaido, H. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  6. The positive transcription factor of the 5S RNA gene induces a 5S DNA-specific gyration in Xenopus oocyte extracts. Kmiec, E.B., Worcel, A. Cell (1985) [Pubmed]
  7. Torsional stress promotes the DNAase I sensitivity of active genes. Villeponteau, B., Lundell, M., Martinson, H. Cell (1984) [Pubmed]
  8. Gene expression changes triggered by exposure of Haemophilus influenzae to novobiocin or ciprofloxacin: combined transcription and translation analysis. Gmuender, H., Kuratli, K., Di Padova K, n.u.l.l., Gray, C.P., Keck, W., Evers, S. Genome Res. (2001) [Pubmed]
  9. Phase I and pharmacologic study of the alkylating agent modulator novobiocin in combination with high-dose chemotherapy for the treatment of metastatic breast cancer. Kennedy, M.J., Armstrong, D.K., Huelskamp, A.M., Ohly, K., Clarke, B.V., Colvin, O.M., Grochow, L.B., Chen, T.L., Davidson, N.E. J. Clin. Oncol. (1995) [Pubmed]
  10. Nuclear DNA polymerases of human carcinomas. DePhilip, R.M., Lynch, W.E., Lieberman, I. Cancer Res. (1977) [Pubmed]
  11. Formation of proinsulin by immobilized Bacillus subtilis. Mosbach, K., Birnbaum, S., Hardy, K., Davies, J., Bülow, L. Nature (1983) [Pubmed]
  12. Inhibitor evidence for allosteric interaction in the replitase multienzyme complex. veer Reddy, G.P., Pardee, A.B. Nature (1983) [Pubmed]
  13. Nalidixic acid, oxolinic acid, and novobiocin inhibit yeast glycyl- and leucyl-transfer RNA synthetases. Wright, H.T., Nurse, K.C., Goldstein, D.J. Science (1981) [Pubmed]
  14. Novobiocin inhibits passive chromatin assembly in vitro. Sealy, L., Cotten, M., Chalkley, R. EMBO J. (1986) [Pubmed]
  15. Novobiocin and related coumarins and depletion of heat shock protein 90-dependent signaling proteins. Marcu, M.G., Schulte, T.W., Neckers, L. J. Natl. Cancer Inst. (2000) [Pubmed]
  16. Illegitimate recombination mediated by calf thymus DNA topoisomerase II in vitro. Bae, Y.S., Kawasaki, I., Ikeda, H., Liu, L.F. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  17. Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase. Gellert, M., O'Dea, M.H., Itoh, T., Tomizawa, J. Proc. Natl. Acad. Sci. U.S.A. (1976) [Pubmed]
  18. Destabilization of the non-pathogenic, cellular prion-protein by a small molecular drug. Ochel, H.J., Gademann, G. Antivir. Ther. (Lond.) (2004) [Pubmed]
  19. Development and characterization of a WEHI-3B D+ monomyelocytic leukemia cell line resistant to novobiocin and cross-resistant to other topoisomerase II-targeted drugs. Rappa, G., Lorico, A., Sartorelli, A.C. Cancer Res. (1992) [Pubmed]
  20. Role of DNA gyrase subunits in synthesis of bacteriophage phi X174 viral DNA. Hamatake, R.K., Mukai, R., Hayashi, M. Proc. Natl. Acad. Sci. U.S.A. (1981) [Pubmed]
  21. Transcription of oxygen-regulated photosynthetic genes requires DNA gyrase in Rhodobacter capsulatus. Zhu, Y.S., Hearst, J.E. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  22. Requirement of a plasmid-encoded protein for replication in vitro of plasmid R6K. Inuzuka, M., Helinski, D.R. Proc. Natl. Acad. Sci. U.S.A. (1978) [Pubmed]
  23. Novobiocin, nalidixic acid, etoposide, and 4'-(9-acridinylamino)methanesulfon-m-anisidide effects on G2 and mitotic Chinese hamster ovary cell progression. Rowley, R., Kort, L. Cancer Res. (1989) [Pubmed]
  24. Alteration of differentiation state of human hepatocytes cultured with novobiocin and butyrate. Kaneko, Y., Nakayama, T., Tsukamoto, A., Kurokawa, K. Cancer Res. (1990) [Pubmed]
  25. Transcriptional coupling between the divergent promoters of a prototypic LysR-type regulatory system, the ilvYC operon of Escherichia coli. Rhee, K.Y., Opel, M., Ito, E., Hung, S., Arfin, S.M., Hatfield, G.W. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  26. Stability of the Peutz-Jeghers syndrome kinase LKB1 requires its binding to the molecular chaperones Hsp90/Cdc37. Nony, P., Gaude, H., Rossel, M., Fournier, L., Rouault, J.P., Billaud, M. Oncogene (2003) [Pubmed]
  27. Expression and analysis of two gyrB genes from the novobiocin producer, Streptomyces sphaeroides. Thiara, A.S., Cundliffe, E. Mol. Microbiol. (1993) [Pubmed]
  28. Mutual suppression of mukB and seqA phenotypes might arise from their opposing influences on the Escherichia coli nucleoid structure. Weitao, T., Nordström, K., Dasgupta, S. Mol. Microbiol. (1999) [Pubmed]
  29. The site-specific recombination system regulating expression of the type 1 fimbrial subunit gene of Escherichia coli is sensitive to changes in DNA supercoiling. Dove, S.L., Dorman, C.J. Mol. Microbiol. (1994) [Pubmed]
  30. Novobiocin- and phorbol-12-myristate-13-acetate-induced differentiation of human leukemia cells associated with a reduction in topoisomerase II activity. Constantinou, A., Henning-Chubb, C., Huberman, E. Cancer Res. (1989) [Pubmed]
  31. Direct inhibition of u.v.-induced DNA excision repair in human cells by novobiocin, coumermycin and nalidixic acid. Dresler, S.L., Robinson-Hill, R.M. Carcinogenesis (1987) [Pubmed]
  32. The entropic penalty of ordered water accounts for weaker binding of the antibiotic novobiocin to a resistant mutant of DNA gyrase: a thermodynamic and crystallographic study. Holdgate, G.A., Tunnicliffe, A., Ward, W.H., Weston, S.A., Rosenbrock, G., Barth, P.T., Taylor, I.W., Pauptit, R.A., Timms, D. Biochemistry (1997) [Pubmed]
WikiGenes - Universities