Disease relevance of motA

• Nucleotide sequence and control of transcription of the bacteriophage T4 motA regulatory gene [1].
• Finally, the transposon insert strains were screened on the TabG Escherichia coli strain, which inhibits the growth of T4 motA mutants, and a motA transposon insert strain was found [2].

High impact information on motA

• Major RNA 5' ends, 900 and 200 bases upstream of uvsX, show homology with previously deduced T4 transcription sites dependent on the T4 transcription factor motA (Guild, N., Gayle, M., Sweeney, R., Hollingsworth, T., Modeer, T., and Gold, L. (1988) J. Mol. Biol. 199, 241-258) [3].
• DNase I footprinting of Esigma(70) and Esigma(70)AsiA on motA and mrh.2 shows extended contacts between the holoenzyme with or without AsiA and upstream regions of these promoters [4].
• Potassium permanganate reactivity shows that the two structure elements are not equivalent: with AsiA, the motA UP element-deleted promoter opens more slowly whereas the motA TC promoter opens like the wild type [4].
• This regB-mediated translation inhibition is not accompanied by an important modification in motA mRNA chemical half-life [5].
• To ensure the complete absence of MotA protein, we deleted the motA gene from the T4 genome [6].

Biological context of motA

• The third is a motA-dependent (middle) promoter which has an unusual CCCGCTT box at -33 [7].
• The motA delta mutation also reduced the replication of an ori(uvsY)-containing plasmid and virtually abolished replication of an ori(34)-containing plasmid [6].

Other interactions of motA

• We show that this rapid synthesis depends on a functional motA gene for rIIB, but not for rIIA, RNA synthesis [7].

References