Disease relevance of S13_08

• Recessive mutations in the slyD (sensitivity to lysis) gene were isolated by selecting for survival after induction of the cloned lysis gene E of bacteriophage phi X174 [1] [1].
• A cloning vector, pUH89, allowing positive selection of recombinant Escherichia coli clones by insertional inactivation of the modified lysis gene E of bacteriophage phi X174, was developed [2].
• Positive-selection vector with enhanced lytic potential based on a variant of phi X174 phage gene E [2].
• RNA polymerase holoenzyme from Micrococcus luteus synthesizes in vitro a run-off transcript of 85 nucleotides from a DNA fragment containing part of gene E of bacteriophage phi X174 [3].
• Cell lysis of Gram-negative bacteria can be efficiently achieved by expression of the cloned lysis gene E of bacteriophage PhiX174 [4].

High impact information on S13_08

• The G4 overlapping gene E is longer than in phiX174 and extends beyond gene D. Sixteen nucleotides at the end of genes D and E in phiX174 are duplicated in G4 before gene J [5].
• On S13 replicative form DNA there are three major binding sites, and the locations correlate with promoter sites at the beginning of genes a and B and a site overlapping gene D and the beginning of gene E. Two less definite binding sites have been localized, one in gene F and one at the gene G-H junction [6].
• In combination with the phaCAB genes, expression of cloned lysis gene E of bacteriophage PhiX174 from plasmid pSH2 has been used to release PHB granules produced in E. coli [7].

Biological context of S13_08

• Translational efficiency of phi X174 lysis gene E is unaffected by upstream translation of the overlapping gene D reading frame [8].

Analytical, diagnostic and therapeutic context of S13_08

• Ten unique cloning sites were introduced into gene E by site-directed mutagenesis [2].

References