Disease relevance of HK022p44

• We have studied the synthesis and stability of cII protein with two experimental systems, phage infection and a cII-producing plasmid [1].
• When CII protein is provided by a prophage, galactokinase is synthesized at a rate dependent on the concentration of CII protein [2].

High impact information on HK022p44

• The tetrameric cII protein from bacteriophage lambda activates transcription from the phage promoters P(RE), P(I), and P(AQ) by binding to two direct repeats that flank the promoter -35 element [3].
• Cleavage of the cII protein of phage lambda by purified HflA protease: control of the switch between lysis and lysogeny [4].
• Six point mutations in the repeated T-T-G-C sequences that flank the -35 consensus region of PRE decreased the apparent affinity of the promoter for cII protein by factors of 4-16 relative to the wild-type affinity [5].
• Thus low concentrations of guanidine hydrochloride promote a more structured conformation of the CII protein [6].
• The recombinant CII protein has been purified to homogeneity by ammonium sulfate fractionation followed by two steps of ion-exchange chromatography [7].

Biological context of HK022p44

• There was no apparent difference between the lacI vs. cII target genes of the shuttle vector in either the background or DMN-induced mutant frequencies [8].
• In the present report, we reveal the molecular nature of the mutations induced by quinoline in the lambda cII gene, which is also a phenotypically selectable marker in the lambda transgene [9].
• Our previous studies indicated that the impaired lysogenisation of the rpoA341 host is due to a defect in transcriptional activation by the phage CII protein and suggested a role for alphaCTD in this process [10].

Associations of HK022p44 with chemical compounds

• On the other hand, although cyclic AMP is required for efficient lysogeny, it did not appear to influence the synthesis, stability, or activity of cII protein [11].

References