High impact information on Cyp4b1

• Anti-Cyp4b1 antibody efficiently inhibited DCB bioactivation by mouse renal microsomes with a high Cyp4b1 content [1].
• Cytochrome P450 4B1 activity was assessed by the cytotoxicity of 4-ipomeanol, a known substrate for P450 4B1 and a model compound for chemical-induced injury to the lung [2].
• The Cyp4b1 structural gene comprises 12 exons that span approximately 23kb [3].
• Cyp4b1 mRNA expression was found restricted to kidney and androgenic up-regulation in several mouse strains was observed [3].
• Transient transfection studies with different Cyp4b1 promoter constructs indicated that this flanking region exhibits promoter activity when expressed in different cell lines and that a 98-truncated fragment represents the minimal sequence required for basal transcription [3].

Biological context of Cyp4b1

• Genotoxic activation of 2-aminofluorene (a marker for Cyp4b1) by male and female mouse kidney microsomes were not affected by the SCD phenotype [4].

Associations of Cyp4b1 with chemical compounds

• Lauric acid, a substrate of Cyp4b1, efficiently inhibited DCB bioactivation by renal and bladder microsomes of the mouse and by purified Cyp4b1 [1].

Other interactions of Cyp4b1

• Two previously identified forms of cytochrome P450 2B, 2B-B1 and 2B-B2, are not detected in rabbit kidney. cDNA encoding cytochrome P450 4B1 was also cloned from the kidney library and found to be identical in sequence to cDNAs cloned from rabbit hepatic and pulmonary libraries [5].

References