Disease relevance of Mapre1

• Here we demonstrate that EB1 protein levels are increased during axon but not dendrite formation in differentiated N2A neuroblastoma cells, and that EB1 localises to microtubule tips throughout extending neurites in these cells [1].

High impact information on Mapre1

• A carboxy-terminal APC-binding fragment of EB1 (EB1-C) functioned as a dominant-negative inhibitor of microtubule stabilization induced by LPA or active mDia [2].
• Knockdown of EB1 with small interfering RNAs also prevented microtubule stabilization [2].
• These results show an evolutionarily conserved pathway for microtubule capture, and suggest that mDia functions as a scaffold protein for EB1 and APC to stabilize microtubules and promote cell migration [2].
• EB1 is a microtubule associated protein which interacts with the APC tumour suppressor protein and components of the cytoplasmic dynein/dynactin complex [1].
• EB1 identifies sites of microtubule polymerisation during neurite development [1].

Biological context of Mapre1

• Monoclonal antibody EB1 has been shown to enhance the actions of hGH on growth and body composition in Snell dwarf mice and to increase food conversion efficiency [3].

Other interactions of Mapre1

• Binding and localization studies showed that EB1, APC and mDia may form a complex at stable microtubule ends [2].

References