Disease relevance of UL24

• Characterization of the bovine herpesvirus 1 homolog of the herpes simplex virus 1 UL24 open reading frame [1].
• The predicted amino acid sequence of this ORF exhibits an overall identity of 38.1% with that of the herpes simplex virus type 1 (HSV1) UL24 ORF as well as an 80.7% identity with the 57-amino-acid consensus sequence found in the UL24-like ORFs of eight herpesviruses (Jacobson et al., J. Virol, 49, 947-959, 1989) [1].
• In HSV-1, the UL26 and UL26.5 open reading frames encode scaffold proteins upon which viral capsids are assembled [2].
• The proteins expressed from the BHV-1 UL26 and UL26.5 homologs facilitated the formation of hybrid type B capsids indistinguishable from those formed entirely with HSV-1-encoded proteins [2].
• We determined the nucleotide sequence of a 3.5-kb region of the bovine herpesvirus 1 (BHV-1) genome which contained the complete BHV-1 homologs of the herpes simplex virus type 1 (HSV-1) UL26 and UL26.5 genes [2].

High impact information on UL24

• We substituted the proteins encoded by the BHV-1 homologs of the UL26 and UL26.5 open reading frames, expressed in baculovirus, for the corresponding HSV-1 proteins in an in vitro HSV-1 capsid assembly system [2].
• To investigate the role of this gene in virus replication, a BHV-1 UL25 deletion mutant was constructed [3].
• The BHV1 UL24-like ORF is contained within a 5.2-kb RNA transcript initiating within the tk ORF and is the largest in a group of four 3'-coterminal transcripts [1].

Analytical, diagnostic and therapeutic context of UL24

• Two approaches were used to monitor the infection of APC by BHV-1 as follows: (i) detection of viral glycoproteins at the cell surface by immunofluorescence staining and (ii) detection of UL26 transcripts by reverse transcription-PCR [4].

References