Disease relevance of rev

• Characterization of bovine immunodeficiency virus rev cDNAs and identification and subcellular localization of the Rev protein [1].
• A cDNA clone was isolated from CAEV infected cells and shown to encode the 18-kDa Rev protein of CAEV [2].
• The rev gene of visna virus is required for productive infection [3].
• The documented requirement for a functional rev gene in productive infections establishes the essential role of this gene in the replication of an animal lentivirus [3].
• The visna virus Rev protein was shown to interact specifically with the genetically defined RRE-V in vitro but was unable to bind the HIV-1 RRE [4].

High impact information on rev

• Deletions and hypervariability in the 5' part of the env gene have implications for the size of the proposed CAEV-1GA Rev protein and the encoded surface glycoprotein (SU) [5].
• To assess the role of the animal lentivirus rev regulatory gene product in the life cycle of visna virus, we introduced mutations into the functional fourth exon of the rev gene of visna virus [3].
• Analysis of the gag and rev proteins in the transfected cells demonstrated that these proteins were not detectable in cells transfected with the tat mutants but could be readily detected when the mutations were complemented in trans with a tat expression vector [6].

Anatomical context of rev

• Cultured cells transfected with a full-length clone of visna DNA produce infectious virus but visna DNA with mutations in rev does not [3].

Analytical, diagnostic and therapeutic context of rev

• Thus, to determine the location of the rev ORF cDNA clones were obtained by PCR amplification of the mRNA from infected cells [7].

References