Disease relevance of Hemt1

• Characterization of the rhodobacter sphaeroides 5-aminolaevulinic acid synthase isoenzymes, HemA and HemT, isolated from recombinant Escherichia coli [1].

High impact information on Hemt1

• A third type of HemT transcript is expressed only in testis [2].
• HemT-3, an alternative transcript of mouse gene HemT specific to male germ cells [3].
• Ultraviolet-visible spectroscopy with HemA suggested the presence of an aldimine linkage between the enzyme and pyridoxal 5'-phosphate that was not observed when HemT was incubated with the cofactor [1].

Analytical, diagnostic and therapeutic context of Hemt1

• Using Northern blot, RT-PCR and in situ hybridization, we show that HemT encodes three different transcripts [2].
• We have now isolated a third HemT transcript, HemT-3, that is found only in testis by Northern blot analysis and RT-PCR [3].

References