Disease relevance of DMXL1

• X-like and Y-like ganglion cells in the Necturus retina [1].
• By digestion of genomic DNA from strain IVICPb 73 (ATCC 32071), we have constructed a DNA library with an insert size average of 8 kb in Escherichia coli XL1 Blue. We have fully sequenced 7 clones comprising 51,022 bp which represent 20 putative protein-coding sequences (seven of them, partial) and one tRNA [2].
• Isolation and characterization of a new extracellular bacteriolytic endopeptidase of Lysobacter sp. XL1 [3].

High impact information on DMXL1

• The DMXL1 gene is located on chromosome 5q22 as determined by radiation hybrid mapping and fluorescence in situ hybridization [4].
• The human DMXL1 gene codes for a large mRNA of 11 kb with an open reading frame of 3027 amino acids [4].
• The recombinant plasmid was transformed into XL1-blue [5].
• The 14 Mb deleted region contains about 60 genes but, with the possible exception of FBN2 and DMXL1, there are no obvious candidate genes for the specific components of the phenotype [6].
• This chapter describes three different methods for random mutagenesis, the use of XL1-red cells, error-prone polymerase chain reaction (PCR), and degenerate oligonucleotides-Pfu (DOP) [7].

References