Disease relevance of Necturus

• When intracellular electrophysiological recordings were obtained from the perfused retina-eyecup preparation of the mud-puppy (Necturus maculosus), the addition of 2-amino-4-phosphonobutyric acid to the bathing medium blocked all responses in the ON channel but left intact the OFF responses including OFF ganglion cell discharge [1].
• Indeed, we identified the authentic thyroid hormone receptors of both alpha and beta types in two perennibranchiate species Necturus maculosus and Proteus anguinus [2].

High impact information on Necturus

• Identification of a distinct synaptic glutamate receptor on horizontal cells in mudpuppy retina [3].
• Amacrine cells in Necturus retina: evidence for independent gamma-aminobutyric acid- and glycine-releasing neurons [4].
• METHODS: Chambered Necturus antral mucosa was perfused with Ringer's/95% O2-5% CO2 at pH 7.25 [5].
• Regulation of intracellular pH (pHi) and its maintenance within physiological ranges during exposure to luminal acid was studied in isolated Necturus duodenal mucosa using liquid sensor microelectrodes [6].
• Effects of aspirin on pathways of ion permeation in Necturus antrum: role of nutrient HCO3 [7].

Chemical compound and disease context of Necturus

• Synaptic organization and ionic basis of on and off channels in mudpuppy retina. II. Chloride-dependent ganglion cell mechanisms [8].
• 1. The surface chemosensitivity to iontophoretically applied acetylcholine (ACh) of single nerve cells in the cardiac ganglion of the mudpuppy was examined [9].
• Physiological and pharmacological basis of GABA and glycine action on neurons of mudpuppy retina. II. Amacrine and ganglion cells [10].
• Experiments were performed in the superfused retina-eyecup of mudpuppies using intracellular electrophysiological techniques to evaluate the effects of serine on amacrine and ganglion cells [11].
• Pituitary adenylate cyclase-activating polypeptide innervation of the mudpuppy cardiac ganglion [12].

Biological context of Necturus

• Intracellular microelectrode techniques were used to determine the effects of luminal aspirin on epithelial cell membrane potentials of Necturus antral mucosa [13].
• Effects of luminal hyperosmolality on cellular and paracellular ion transport pathways in necturus antrum [14].
• Previous studies have shown that mudpuppy taste receptor cells respond to sour taste stimuli (weak acids) with depolarizing receptor potentials or action potentials that are blocked by the K+ channel blocker tetraethylammonium [15].
• Synaptic transmission at N-methyl-D-aspartate receptors in the proximal retina of the mudpuppy [16].
• Heart tissue (atrium or the entire heart) from all the animals studied (trout, mudpuppy, bullfrog tadpole, and adult bullfrog) responded to isoproterenol in a dose-dependent fashion with increased heart rate and contractile force [17].

Anatomical context of Necturus

• Exogenous surface-active phospholipid protects Necturus gastric mucosa against luminal acid and barrier-breaking agents [18].
• We have purified a protein from Necturus maculosus gallbladder cells that forms chloride channels in an artificial membrane [19].
• Previous studies have shown that, immediately after the addition of galactose or alanine to the solution bathing the mucosal surface of Necturus small intestine, there is a rapid depolarization of the electrical potential difference across the mucosal membrane (psi mc) [20].
• This study examined the role of Ca(2+) in regulatory volume decrease by Necturus erythrocytes [21].
• The mechanisms of apparent streaming potentials elicited across Necturus gallbladder epithelium by addition or removal of sucrose from the apical bathing solution were studied by assessing the time courses of: (a) the change in transepithelial voltage (Vms) [22].

Associations of Necturus with chemical compounds

• Average pHi in Necturus cells in CO2/HCO3-buffered Ringer's solution was approximately 7 [23].
• Average beta i = 28 mM per pH unit in Necturus and 42 mM per pH unit in rabbit cells in HEPES-buffered Ringer's solution [23].
• Gastric HCO-3 transport was studied in Rana temporaria fundus, in which H+ secretion had been inhibited with the histamine H2-receptor antagonists metiamide or cimetidine (10(-3) M), and in Necturus antrum, which secreted HCO-3 spontaneously [24].
• Do N-methyl-D-aspartate receptors mediate synaptic responses in the mudpuppy retina [25]?
• The role of excitatory amino acid transmitters in the mudpuppy retina: an analysis with kainic acid and N-methyl aspartate [26].

Gene context of Necturus

• The percent identity between bird sequences and either mouse or mudpuppy was lower (79%); the mudpuppy AHR was 74% identical to the mouse AHR [27].
• We localized gap junctions in the retinas of rat, goldfish, and mudpuppy by using antisera directed against proteins that make up the connexon channels in two tissues from which connexins have been isolated: liver (connexin 32; CX32) and heart (connexin 43; CX43) [28].
• A specific androgen-binding protein (ABP) in Necturus testis and its zonal distribution [29].
• In contrast, CFTR from the amphibians Necturus maculosus and Xenopus laevis (XCFTR) can be activated to similar degrees by stimulation of either PKA or PKC [30].
• Characteristics of the galanin-induced depolarization of mudpuppy parasympathetic postganglionic neurons [31].

Analytical, diagnostic and therapeutic context of Necturus

• 1. Characteristics of the membrane currents activated by arachidonic acid (AA) in dissociated mudpuppy parasympathetic neurons have been determined using the perforated-patch whole cell recording technique [32].
• Immunocytochemistry revealed serotonin-like immunostaining in cells in mammalian taste buds and Merkel-like basal cells in taste buds of mudpuppies [33].
• Regulation of intracellular chloride activity during perfusion with hypertonic solutions in the Necturus proximal tubule [34].
• Micropuncture study of the renal responses of the urodele amphibian Necturus maculosus to injections of arginine vasotocin and an anti-aldosterone compound [35].
• The presence of glutamate immunoreactivity (glu-IR) in the nerve fibers of the mudpuppy taste bud was investigated by electron microscopy [36].

References