High impact information on Nfyb

• In contrast, the nuclear import of NF-YB and NF-YC requires dimer formation [1].
• NF-Y is composed of three subunits, NF-YA, NF-YB, and NF-YC, all required for DNA binding [2].
• The mouse NF-YB also associates with OsMADS18 in vivo and in vitro as a NF-YB-NF-YC dimer [3].
• Reduction of NF-YB by dithiothreitol (DTT) was essential for reconstitution of specific NF-Y CCAAT box DNA binding activity in vitro [4].
• DTT was required, however, for the functional association of NF-YC with wild-type NF-YB but not with the NF-YB cysteine mutants [4].

Biological context of Nfyb

• NF-YB is assigned to human chromosome 12 and to mouse chromosome 10 [5].
• We demonstrated by transfections of GAL4 fusion constructs, that mouse NF-YB does not contain activation domains while the C-terminal part of SMNF-YB has transcriptional activation potential [6].
• This study provides direct evidence of the identification of NF-YA and NF-YB as the previously described factors A and B. Moreover, these results strongly implicate NF-Y in the expression of the MHC class II gene I-A beta [7].

Associations of Nfyb with chemical compounds

• NF-YB mutants in which the highly conserved cysteine residues at positions 85 and 89 had been converted to serines existed only as monomers and did not require DTT for functional NF-Y DNA binding activity [4].

Other interactions of Nfyb

• A marked decrease of DeltaNp73, NF-YB and p300 was detected 6 hours after serum stimulation when the expression of PDGFRB decreased [8].

Analytical, diagnostic and therapeutic context of Nfyb

• We performed chromatin immunoprecipitation with antibodies against p73, DeltaNp73, NFYB, p300 and HDAC1 at different periods after serum stimulation in serum-starved NIH3T3 cells [8].

References