Disease relevance of CSTF2T

• The gene CSTF2T, encoding the human variant CstF-64 polyadenylation protein tauCstF-64, lacks introns and may be associated with male sterility [1].

High impact information on CSTF2T

• The mouse tauCstF-64 protein fits the criteria of the variant CstF-64, including antibody reactivity, size, germ cell expression, and a common proteolytic digest pattern with tauCstF-64 from testis [2].
• We have named the variant form "tau CstF-64," and we describe here the cloning and characterization of the mouse tauCstF-64 cDNA, which maps to chromosome 19 [2].
• Alignment of human tauCstF-64 with human genome sequence from chromosome 10 shows that CSTF2T lacks introns [1].
• Recent research in our laboratory has uncovered a new form of an essential polyadenylation protein, tauCstF-64, that is most highly expressed in male germ cells, and to a smaller extent in the brain, and which we propose plays a significant role in AAUAAA-independent mRNA polyadenylation in germ cells [3].
• Polyadenylation proteins CstF-64 and tauCstF-64 exhibit differential binding affinities for RNA polymers [4].

Biological context of CSTF2T

• This supports the hypothesis that tauCstF-64 promotes germ-cell-specific patterns of polyadenylation by binding to different downstream sequence elements [4].
• Therefore a paralogous variant, tauCstF-64 is expressed in male germ cells to maintain normal spermatogenesis [4].

Other interactions of CSTF2T

• We quantified K(d) values of CstF-64 and tauCstF-64 RBDs for various ribopolymers using an RNA cross-linking assay [4].

Analytical, diagnostic and therapeutic context of CSTF2T

• Radiation hybrid mapping places the human tauCstF-64 gene at 10q22-q23, which is the site of a translocation that has been associated with human neurological problems and male infertility [1].

References