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Gene Review:

hypX - HypX

Ralstonia eutropha H16

Rey, L. et al., Bleijlevens, B. et al., Buhrke, T. et al.

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Disease relevance of hypX

An open reading frame (designated hypX) encoding a protein with a calculated M(r) of 62300 that exhibits extensive sequence similarity with HoxX from Alcaligenes eutrophus (52% identity) and Bradyrhizobium japonicum (57% identity) was identified 10 bp downstream of hypE [1].
The hydrogenase gene cluster of Rhizobium leguminosarum bv. viciae contains an additional gene (hypX), which encodes a protein with sequence similarity to the N10-formyltetrahydrofolate-dependent enzyme family and is required for nickel-dependent hydrogenase processing and activity [1].
The hypX gene of the facultative lithoautotrophic bacterium Ralstonia eutropha is part of a cassette of accessory genes (the hyp cluster) required for the proper assembly of the active site of the [NiFe]-hydrogenases in the bacterium [2].

High impact information on hypX

hoxX (hypX) is a functional member of the Alcaligenes eutrophus hyp gene cluster [3].
These similarities strongly suggest that HypX/HoxX have dual functions: binding of the C1 donor N10-formyltetrahydrofolate and transfer of the C1 to an unknown substrate, and catalysis of a reaction involving polarization of the C = O bond of an X-CO-SCoA substrate [1].

References

The hydrogenase gene cluster of Rhizobium leguminosarum bv. viciae contains an additional gene (hypX), which encodes a protein with sequence similarity to the N10-formyltetrahydrofolate-dependent enzyme family and is required for nickel-dependent hydrogenase processing and activity. Rey, L., Fernández, D., Brito, B., Hernando, Y., Palacios, J.M., Imperial, J., Ruiz-Argüeso, T. Mol. Gen. Genet. (1996) [Pubmed]
The auxiliary protein HypX provides oxygen tolerance to the soluble [NiFe]-hydrogenase of ralstonia eutropha H16 by way of a cyanide ligand to nickel. Bleijlevens, B., Buhrke, T., van der Linden, E., Friedrich, B., Albracht, S.P. J. Biol. Chem. (2004) [Pubmed]
hoxX (hypX) is a functional member of the Alcaligenes eutrophus hyp gene cluster. Buhrke, T., Friedrich, B. Arch. Microbiol. (1998) [Pubmed]

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