Disease relevance of putA

• Enzymatic properties of the purified putA protein from Salmonella typhimurium [1].
• Nucleotide sequence of regulatory region (R & R) with putA gene (EMBL Accession No. U39227) from Photobacterium leiognathi PL741 has been determined, and the putA gene encoded amino acid sequence of proline dehydrogenase is deduced [2].
• These observations were exploited in preparing an otherwise isogenic set of Escherichia coli K-12 strains with well-defined defects in the putP and putA genes [3].

High impact information on putA

• The proline utilization (put) operon from Salmonella typhimurium consists of the putP gene, encoding a proline transporter, and the putA gene, encoding an enzyme with both proline dehydrogenase and 1-pyrroline-5-carboxylate dehydrogenase activities [4].
• We report the isolation of super-repressor mutants of PutA that decrease expression from the putA promoter in the presence or absence of proline [4].
• Purification of the putA gene product. A bifunctional membrane-bound protein from Salmonella typhimurium responsible for the two-step oxidation of proline to glutamate [5].
• DNA sequence of the putA gene from Salmonella typhimurium: a bifunctional membrane-associated dehydrogenase that binds DNA [6].
• These mutants were selected using two phenotypes: the ability to degrade a toxic proline analogue, dehydroproline, due to overexpression of putA enzyme activity, or overexpression of lacZ from put::Mud operon fusions [7].

Biological context of putA

• The 420-bp put control region contains the putP and putA promoters, multiple operator sites, multiple catabolite repression protein binding sites, and several potential integration host factor (IHF)-binding sites (ihf) [8].

Associations of putA with chemical compounds

• In the previous paper (Menzel, R., and Roth, J. (1981) J. Biol. Chem. 256, 9755-9761) we have described the purification of a protein, the putA gene product, which has both proline oxidase and pyrroline-5-carboxylic acid dehydrogenase activities [1].

Regulatory relationships of putA

• Genetic evidence suggests that putA protein autogenously represses transcription of the putA and putP genes [7].

References