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Gene Review:

PRM2 - protamine 2

Bos taurus

Lee, D.R. et al., Kremling, H. et al., Maier, W.M. et al.

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High impact information on PRM2

A second protamine variant, protamine P2, has to date been isolated only from human and murine spermatozoa where it represents the major fraction of basic nuclear protein [1].
At 2, 5, and 10 wk, cultured cells were examined and evaluated by reverse transcription-polymerase chain reaction (RT-PCR) and Northern blot analysis for protamine-2 (PRM-2) and transition protein-1 (TP-1) mRNA, expressed specifically in round spermatids [2].
Furthermore, primer extension experiments on bull PRM2 mRNA and sequencing of junction fragments revealed alternative splicing of mRNA resulting in two putative isoforms of the protein [3].
As compared to the PRM2 genes of man, mouse and rat the bovine gene lacks a highly conserved sequence coding for the amino acids RLHRIH [3].

Anatomical context of PRM2

Following RT-PCR and Northern blot analysis, specific bands corresponding to PRM-2 and TP-1 were detected only in adult testis RNA or after 10 wk of culture [2].

References

The lack of protamine 2 (P2) in boar and bull spermatozoa is due to mutations within the P2 gene. Maier, W.M., Nussbaum, G., Domenjoud, L., Klemm, U., Engel, W. Nucleic Acids Res. (1990) [Pubmed]
In vitro production of haploid germ cells from fresh or frozen-thawed testicular cells of neonatal bulls. Lee, D.R., Kaproth, M.T., Parks, J.E. Biol. Reprod. (2001) [Pubmed]
The bovine protamine 2 gene: evidence for alternative splicing. Kremling, H., Reinhart, N., Schlösser, M., Engel, W. Biochim. Biophys. Acta (1992) [Pubmed]

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