High impact information on ANXA8

• Immunofluorescence localized annexin VIII largely to the chondrocyte cell membrane [1].
• Northern and Western blotting confirmed that annexin VIII was expressed by growth plate chondrocytes and not by epiphyseal chondrocytes [1].
• In OA cartilage, the protein was located in a subset of mid- to deep zone chondrocytes and in the matrix surrounding these cells; no annexin VIII was detected in normal articular cartilage [1].
• A control experiment using phospholipid (phosphatidylserine/phosphatidylglycerol/phosphtidylcholine) liposome-Sepharose 4B revealed that annexins III, IV and V were the Ca(2+)-dependent proteins binding to the column in the lung soluble fractions, while annexin VIII was not detected [2].

Anatomical context of ANXA8

• Thus annexin VIII is a marker for chondrocyte differentiation during normal endochondral ossification and may act as a marker for cells undergoing inappropriate differentiation in OA [1].
• One of the cDNA clones identified encoded the bovine orthologue of annexin VIII, a protein not previously described in the growth plate [1].
• In a preliminary study, we examined the distribution of annexin VIII in normal and osteoarthritic (OA) articular cartilage [1].

Analytical, diagnostic and therapeutic context of ANXA8

• Immunohistochemistry of the fetal bovine growth plate identified a gradient of increasing annexin VIII protein from the proliferative to the hypertrophic zone [1].

References