High impact information on Smtn

• In vitro dot blot assays, in which immobilized actin was overlaid with radio-labeled smoothelin, showed binding of smoothelin-A to actin filaments, but not to monomeric G-actin [1].
• The two major isoforms of smoothelin (A and B) contain a calponin homology (CH) domain, colocalize with alpha-smooth muscle actin (alpha-SMA) in stress fibers and are only expressed in contractile smooth muscle cells (SMCs) [1].
• A truncated smoothelin, containing the calponin homology domain, associated with stress fibers when transfected and bound to actin filaments in overlay, but to a lesser extent [1].
• We also identified several genes involved with stress responses (cathepsin H, UBC2E, IGFBP3, smoothelin) [2].
• Immunocytochemistry demonstrated both smooth muscle myosin heavy chain and smooth muscle alpha-actin in the prostatic SMC 21 days after castration, whereas RT-PCR amplified the message for smoothelin [3].

Anatomical context of Smtn

• Our results indicate an interaction between smoothelin and actin filaments [1].
• Subsequently 1E12 antigen was expressed in coronary artery wall in ED18 hearts, and finally smoothelin [4].

Analytical, diagnostic and therapeutic context of Smtn

• ELISA results showed that the binding of smoothelin to actin has no significant isoform specificity [1].

References