Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Gene Review:

fliA - flagellar biosynthesis sigma factor

Legionella pneumophila str. Paris

Heuner, K. et al., Molofsky, A.B. et al.

Heuner, Dietrich, Skriwan, Steinert, Hacker,

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Disease relevance of fliA

The fliA gene of Legionella pneumophila encoding the alternative sigma(28) factor was inactivated by introducing a kanamycin resistance cassette [1].

High impact information on fliA

To test how particular components of the L. pneumophila flagellar regulon contribute to virulence, we compared a fliA mutant with strains whose flagellar construction is disrupted at various stages [2].
Reporter gene assays indicated that the flaA promoter is not active in the fliA mutant strain [1].

Analytical, diagnostic and therapeutic context of fliA

Electron microscopy and Western blot analysis revealed that the fliA mutant strain is aflagellate and expresses no flagellin [1].
The fliA mutant strain multiplied less effectively in coculture with amoebae than the wild-type strain and was not able to replicate in coculture with Dictyostelium discoideum [1].

References

Influence of the alternative sigma(28) factor on virulence and flagellum expression of Legionella pneumophila. Heuner, K., Dietrich, C., Skriwan, C., Steinert, M., Hacker, J. Infect. Immun. (2002) [Pubmed]
Components of the Legionella pneumophila flagellar regulon contribute to multiple virulence traits, including lysosome avoidance and macrophage death. Molofsky, A.B., Shetron-Rama, L.M., Swanson, M.S. Infect. Immun. (2005) [Pubmed]

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