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Gene Review:

msp5 - major surface protein 5

Anaplasma marginale str. St. Maries

Knowles, D. et al., Reyna-Bello, A. et al., Torioni de Echaide, S. et al., Molad, T. et al., Ndung'u, L.W. et al., et al.

Knowles, Torioni de Echaide, Palmer, McGuire, Stiller, McElwain,

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Disease relevance of msp5

The Anaplasma marginale msp5 gene encodes a 19-kilodalton protein conserved in all recognized Anaplasma species [1].
An indirect enzyme-linked immunosorbent assay (ELISA) was developed for the serological diagnosis of bovine anaplasmosis with purified recombinant major surface protein 5 (MSP5) of Anaplasma marginale produced in Escherichia coli [2].

High impact information on msp5

Conformational dependence of Anaplasma marginale major surface protein 5 surface-exposed B-cell epitopes [3].
Antibody against an Anaplasma marginale MSP5 epitope common to tick and erythrocyte stages identifies persistently infected cattle [4].
A protein epitope of major surface protein 5 (MSP5), defined by monoclonal antibody (MAb) ANAF16C1, is conserved among Anaplasma species (E. S. Visser, T. C. McGuire, G. H. Palmer, W. C. Davis, V. Shkap, E. Pipano, and D. P. Knowles, Jr., Infect. Immun. 60:5139-5144, 1992) and is expressed in the salivary glands of infected ticks [4].
Anaplasma centrale msp4 and msp5 genes were cloned and sequenced, and the recombinant proteins were expressed [5].
A. centrale msp5 nucleotide sequences shared 86.8% identity with A. marginale msp5, and there was 92.9% homology between A. centrale and A. marginale encoded amino acids of the MSP5 protein [5].

Biological context of msp5

Serum antibody responses against MSP5 were detected in calves experimentally infected with A. marginale as early as 21 days postinfection and reached maximum titers at 28 days postinfection [2].

Anatomical context of msp5

During acute infection, the MSP5 cELISA detected antibodies prior to or concomitantly with the appearance of rickettsiae in erythrocytes [4].

Analytical, diagnostic and therapeutic context of msp5

Third, msp5 sequence analysis showed >95% identity among 30 nPCR amplicons from cattle naturally infected with field strains of A. marginale [6].
The true positive and negative A. marginale infection status of 235 randomly selected cattle was determined by using a nested PCR (nPCR) coupled with msp5 sequence analysis and hybridization [6].
Sera from 149 goats in four regions of Kenya and from 302 goats in six U.S. states were tested for the presence of epitope-specific antibodies with the MSP5 competitive inhibition ELISA [7].
The specificity of the recombinant-MSP5 cELISA within North America was established by using 261 serum samples from cattle in the regions of Hawaii and Northern Ontario where anaplasmosis is not endemic and from cattle proven by splenectomy or subinoculation of whole blood into susceptible splenectomized recipients to be uninfected [4].
Positive results obtained in the MSP5 ELISA were further confirmed by immunoblotting, with the recombinant MSP5 as the antigen [2].

References

The Anaplasma marginale msp5 gene encodes a 19-kilodalton protein conserved in all recognized Anaplasma species. Visser, E.S., McGuire, T.C., Palmer, G.H., Davis, W.C., Shkap, V., Pipano, E., Knowles, D.P. Infect. Immun. (1992) [Pubmed]
Evaluation of an enzyme-linked immunosorbent assay using recombinant major surface protein 5 for serological diagnosis of bovine anaplasmosis in Venezuela. Reyna-Bello, A., Cloeckaert, A., Vizcaíno, N., Gonzatti, M.I., Aso, P.M., Dubray, G., Zygmunt, M.S. Clin. Diagn. Lab. Immunol. (1998) [Pubmed]
Conformational dependence of Anaplasma marginale major surface protein 5 surface-exposed B-cell epitopes. Munodzana, D., McElwain, T.F., Knowles, D.P., Palmer, G.H. Infect. Immun. (1998) [Pubmed]
Antibody against an Anaplasma marginale MSP5 epitope common to tick and erythrocyte stages identifies persistently infected cattle. Knowles, D., Torioni de Echaide, S., Palmer, G., McGuire, T., Stiller, D., McElwain, T. J. Clin. Microbiol. (1996) [Pubmed]
Molecular conservation of MSP4 and MSP5 in Anaplasma marginale and A. centrale vaccine strain. Molad, T., Brayton, K.A., Palmer, G.H., Michaeli, S., Shkap, V. Vet. Microbiol. (2004) [Pubmed]
Detection of cattle naturally infected with Anaplasma marginale in a region of endemicity by nested PCR and a competitive enzyme-linked immunosorbent assay using recombinant major surface protein 5. Torioni de Echaide, S., Knowles, D.P., McGuire, T.C., Palmer, G.H., Suarez, C.E., McElwain, T.F. J. Clin. Microbiol. (1998) [Pubmed]
Detection of Anaplasma ovis infection in goats by major surface protein 5 competitive inhibition enzyme-linked immunosorbent assay. Ndung'u, L.W., Aguirre, C., Rurangirwa, F.R., McElwain, T.F., McGuire, T.C., Knowles, D.P., Palmer, G.H. J. Clin. Microbiol. (1995) [Pubmed]

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