Disease relevance of msp4

• Derivation of the complete msp4 gene sequence of Anaplasma marginale without cloning [1].

High impact information on msp4

• The phylogenies of the msp4 sequences of 13 isolates from Oklahoma in comparison with those of 7 Latin American isolates and 12 U.S. isolates by maximum-parsimony (MP) and maximum-likelihood (ML) analyses, with A. centrale and A. ovis sequences used as outgroups, provided strong bootstrap analysis support for a Latin American clade [2].
• The gene and protein sequences of MSP1a and msp4 nucleotide sequences were used to infer the phylogenetic relationships among Oklahoma and New World isolates from Argentina, Brazil, Mexico, and the United States. All 11 A. marginale isolates collected from Oklahoma had different MSP1a sequences but identical MSP4 sequences [2].
• Phylogenetic studies of A. marginale geographic isolates using msp4 and msp1alpha provide information about the biogeography and evolution of A. marginale: msp1alpha genotypes evolve under positive selection pressure [3].
• Genetic variation among strains of Anaplasma spp. from animals and ticks was characterized using the A. marginale msp1alpha and the Anaplasma spp. msp4 genes [4].
• Anaplasma centrale msp4 and msp5 genes were cloned and sequenced, and the recombinant proteins were expressed [5].

Biological context of msp4

• However, the amino acid sequences of msp4 demonstrated definite phylogeographical relationship [6].
• These results suggest that the amino acid sequences of msp4 should be used for phylogenetic identification of A. marginale strains and may be an important tool for the epidemiology and control of anaplasmosis [6].
• The msp4 gene of an A. marginale strain isolated in Paran , Brazil, was amplified by PCR and sequenced; its cloning into the pET102/D-TOPO vector produced an msp4-6xHis-V5-HP thioredoxin fusion gene construct [7].

Analytical, diagnostic and therapeutic context of msp4

• The PCR products were sequenced to obtain the entire msp4 gene and surrounding regions [1].
• Sequence analysis of the msp4 gene of Anaplasma phagocytophilum strains [8].

References