Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Gene Review:

NP - NP

Mopeia Lassa virus reassortant 29

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Disease relevance of NP

Vaccinia recombinant expressing Lassa-virus internal nucleocapsid protein protects guineapigs against Lassa fever [1].
Expression of the Lassa virus nucleocapsid protein in insect cells infected with a recombinant baculovirus: application to diagnostic assays for Lassa virus infection [2].

High impact information on NP

Clone ML29, selected from a library of MOPV/LASV (MOP/LAS) reassortants, encodes the major antigens (nucleocapsid and glycoprotein) of LASV and the RNA polymerase and zinc-binding protein of MOPV [3].
The Lassa virus nucleocapsid protein gene and segments from it were expressed in Escherichia coli under the control of the lac promoter in pUC-based plasmids [4].

Analytical, diagnostic and therapeutic context of NP

More than 3,100 households in 27 selected villages distributed in the main geographic regions of Guinea were surveyed for the presence of Lassa virus-specific IgG antibodies (LVA), using an enzyme-linked immunosorbent assay (ELISA) with Lassa virus nucleocapsid protein expressed in insect cells infected with a recombinant baculovirus as antigen [5].

References

Vaccinia recombinant expressing Lassa-virus internal nucleocapsid protein protects guineapigs against Lassa fever. Clegg, J.C., Lloyd, G. Lancet (1987) [Pubmed]
Expression of the Lassa virus nucleocapsid protein in insect cells infected with a recombinant baculovirus: application to diagnostic assays for Lassa virus infection. Barber, G.N., Clegg, J.C., Lloyd, G. J. Gen. Virol. (1990) [Pubmed]
A live attenuated vaccine for Lassa fever made by reassortment of Lassa and Mopeia viruses. Lukashevich, I.S., Patterson, J., Carrion, R., Moshkoff, D., Ticer, A., Zapata, J., Brasky, K., Geiger, R., Hubbard, G.B., Bryant, J., Salvato, M.S. J. Virol. (2005) [Pubmed]
Expression of Lassa virus nucleocapsid protein segments in bacteria: purification of high-level expression products and their application in antibody detection. Barber, G.N., Clegg, J.C., Chamberlain, J. Gene (1987) [Pubmed]
Lassa virus activity in Guinea: distribution of human antiviral antibody defined using enzyme-linked immunosorbent assay with recombinant antigen. Lukashevich, L.S., Clegg, J.C., Sidibe, K. J. Med. Virol. (1993) [Pubmed]

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