High impact information on ND5

• The ND5 subunit was labeled by a photoaffinity analogue of fenpyroximate in bovine mitochondrial complex I [1].
• Labeling of the ND5 subunit was stimulated by NADH/NADPH but was prevented by various complex I inhibitors [1].
• These results suggest that the ND5 subunit is involved in construction of the inhibitor- and quinone-binding site(s) [1].
• A multiplex-PCR was evaluated for BPV-2 L1 gene and bovine mitochondrial genome ND5 gene (internal control) detection followed by a second round of BPV-2 amplification by a semi-nested PCR (SN-PCR) [2].
• In addition, mitochondrial DNA ND5 in proportion to a nuclear gene (luteinizing hormone receptor) was determined at the 1st, 2nd, 3rd, 10th, and 15th passages using semi-quantitative PCR amplification [3].

Associations of ND5 with chemical compounds

• These results together with our earlier observation that the amiloride analogues prevent labeling of the ND5 subunit of complex I with a fenpyroximate analogue suggest the involvement of ND5 in H(+) (Na(+)) translocation and no direct involvement of electron carriers in H(+) (Na(+)) translocation [4].

Analytical, diagnostic and therapeutic context of ND5

• Further analysis using the 2D gel electrophoresis implied that the labeled protein was the ND5 subunit [1].

References