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Gene Review:

ilvA - threonine dehydratase

Corynebacterium glutamicum ATCC 13032

Hüser, A.T. et al., Lange, C. et al., Radmacher, E. et al., Colón, G.E. et al.

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Disease relevance of ilvA

Production of isoleucine by overexpression of ilvA in a Corynebacterium lactofermentum threonine producer [1].

High impact information on ilvA

The new pantothenate production strain carries a deletion of the ilvA gene to abolish isoleucine synthesis, the promoter down-mutation P-ilvEM3 to attenuate ilvE gene expression and thereby increase ketoisovalerate availability, and two compatible plasmids to overexpress the ilvBNCD genes and duplicated copies of the panBC operon [2].
The valine-caused isoleucine limitation was supported by the finding that the inhibitory valine effect was linked to the ilvA deletion that results in isoleucine auxotrophy [3].
The same genes, ilvBNCD, overexpressed in an ilvA deletion mutant which is unable to synthesize L-isoleucine increased the concentration of this amino acid to 58 mM [4].

References

Production of isoleucine by overexpression of ilvA in a Corynebacterium lactofermentum threonine producer. Colón, G.E., Nguyen, T.T., Jetten, M.S., Sinskey, A.J., Stephanopoulos, G. Appl. Microbiol. Biotechnol. (1995) [Pubmed]
Rational design of a Corynebacterium glutamicum pantothenate production strain and its characterization by metabolic flux analysis and genome-wide transcriptional profiling. Hüser, A.T., Chassagnole, C., Lindley, N.D., Merkamm, M., Guyonvarch, A., Elisáková, V., Pátek, M., Kalinowski, J., Brune, I., Pühler, A., Tauch, A. Appl. Environ. Microbiol. (2005) [Pubmed]
Global expression profiling and physiological characterization of Corynebacterium glutamicum grown in the presence of L-valine. Lange, C., Rittmann, D., Wendisch, V.F., Bott, M., Sahm, H. Appl. Environ. Microbiol. (2003) [Pubmed]
Linking central metabolism with increased pathway flux: L-valine accumulation by Corynebacterium glutamicum. Radmacher, E., Vaitsikova, A., Burger, U., Krumbach, K., Sahm, H., Eggeling, L. Appl. Environ. Microbiol. (2002) [Pubmed]

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