High impact information on Ndae1

• Our functional data suggest this Na(+) driven anion exchanger (NDAE1) is responsible for the Na(+)-dependent Cl(-)-HCO(3)(-) exchange activity characterized in neurons, kidney, and fibroblasts [1].
• Moreover, our results begin to delineate the NDAE1 topology, i.e., both the NH(2) and COOH termini are intracellular [2].
• In the present study we used immunohistochemical and Western blot techniques to characterize the developmental expression, subcellular localization, and tissue distribution of NDAE1 protein in D. melanogaster [2].
• We have shown that a polyclonal antibody raised against the NH(2) terminus of NDAE1 (alpha CWR57) recognizes NDAE1 electrophysiologically characterized in Xenopus oocytes [2].
• These results suggest that NDAE1 may be a key pH(i) regulatory protein and may contribute to basolateral ion transport in epithelia and nervous system of Drosophila [2].

Anatomical context of Ndae1

• NDAE1 is expressed throughout Drosophila development in the central and peripheral nervous systems, sensilla, and the alimentary tract (Malpighian tubules, gut, and salivary glands) [2].
• Coimmunolabeling of larval tissues with NDAE1 antibody and a monoclonal antibody to the Na(+)-K(+)-ATPase alpha-subunit revealed that the majority of NDAE1 is located at the basolateral membranes of Malpighian tubule cells [2].

References