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Gene Review

EF2  -  Elongation factor 2

Drosophila melanogaster

Synonyms: CG2238, Dmel\CG2238, EF-2, EF-2b, EF2b, ...
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High impact information on Ef2b

  • This identification was based on the high degree of its amino acid sequence identity (greater than 80%) to that of hamster EF2 [1].
  • Conserved sequence segments shared with a variety of GTP binding proteins are found in the amino terminal third of the protein, and segments unique to EF2 and its prokaryotic functional homolog, EF-G, are in the carboxy terminal half; these two regions are segregated in two respective exons [1].
  • The gene encoding Drosophila EF2 is found at position 39E-F of the 2L chromosomal arm and maybe identical to the M(2)H locus, which produces a Minute phenotype when mutated [1].
  • Because DTM fortuitously bears the same point mutation as found in the A chain of CRM197, an ADP-ribosyltransferase (ADPrT)-deficient form of DTx, we hypothesized that the dramatic low-temperature-sensitive effects did not stem from ADP-ribosylation of elongation factor 2 (EF-2) [2].
  • Phylogenies were inferred from both the gene and the protein sequences of the translational elongation factor termed EF-2 (for Archaea and Eukarya) and EF-G (for Bacteria) [3].

Associations of Ef2b with chemical compounds

  • However, the histidyl residue target for ADP-ribosylation of EF-2 by diphtheria toxin is replaced by tyrosine in EFT-1 [4].

Analytical, diagnostic and therapeutic context of Ef2b

  • Sequence analysis of genomic and cDNA clones revealed that the deduced amino acid sequence of the protein (EFT-1) is 38% identical to that of mammalian and Drosophila elongation factor 2 (EF-2) [4].
  • Amplification of fragments encoding highly conserved regions of EF-2 using the polymerase chain reaction led to the isolation of a fragment encoding the modifiable histidyl residue and which likely represents part of the C. elegans EF-2 gene (eft-2) [4].


  1. Isolation and characterization of the Drosophila translational elongation factor 2 gene. Grinblat, Y., Brown, N.H., Kafatos, F.C. Nucleic Acids Res. (1989) [Pubmed]
  2. Characterization of a cloned temperature-sensitive construct of the diphtheria toxin A domain. Lee, J.W., Cho, E., Aghaian, E., Aghaian, E., Der, J., Wisnieski, B.J. Biochemistry (2005) [Pubmed]
  3. Early evolutionary relationships among known life forms inferred from elongation factor EF-2/EF-G sequences: phylogenetic coherence and structure of the archaeal domain. Cammarano, P., Palm, P., Creti, R., Ceccarelli, E., Sanangelantoni, A.M., Tiboni, O. J. Mol. Evol. (1992) [Pubmed]
  4. Isolation and characterization of eft-1, an elongation factor 2-like gene on chromosome III of Caenorhabditis elegans. Ofulue, E.N., Candido, E.P. DNA Cell Biol. (1992) [Pubmed]
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