High impact information on eft-2

• A unique serine-specific elongation factor Tu found in nematode mitochondria [1].
• Xenopus oocyte maturation, the in vivo and in vitro phosphorylation of elongation factor EF-1 are inhibited by olomoucine [2].
• Partial sequencing of a genomic clone encoding the entire C. elegans EF-2 gene (named eft-2) has so far revealed two introns of 48 and 44 bp following codons Gln-191 and Gln-250, respectively [3].
• The deduced amino acid sequence is greater than 80% identical to that of mammalian and Drosophila EF-2 [3].
• Clone pCef6A, encoding the entire C. elegans EF-2 mRNA sequence including 45 nucleotides of 5'-untranslated region, contains a 2,556-bp open reading frame which predicts a polypeptide of 852 amino acid residues (Mr 94,564) [3].

Biological context of eft-2

• A Caenorhabditis elegans lambda ZAP cDNA library was screened using a fragment amplified from highly conserved regions of the mammalian and Drosophila elongation factor 2 (EF-2) [3].

Analytical, diagnostic and therapeutic context of eft-2

• Southern and Northern blot analyses indicate that eft-2 is a single-copy gene and encodes a 3-kb mRNA species which is present throughout nematode development [3].
• Molecular cloning and characterization of the Caenorhabditis elegans elongation factor 2 gene (eft-2) [3].
• Sequence analysis of these clones and comparisons with hamster and Drosophila EF-2 sequences suggests that they encode C. elegans EF-2 [3].

References