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Gene Review:

igdb-1 - Protein IGDB-1

Caenorhabditis elegans

Lei, J. et al., Mohan, S. et al., Russell, M.W. et al., Benian, G.M. et al., Gamble, H.R. et al., et al.

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Disease relevance of fibronectin

We have expressed and purified from Escherichia coli twitchin's protein kinase catalytic core and flanking sequences that do not include fibronectin III and immunoglobulin C2 domains [1].

High impact information on fibronectin

The C. elegans sax-3 gene encodes a predicted transmembrane protein with five immunoglobulin domains and three fibronectin type III repeats that is closely related to Drosophila Robo [2].
UNC-73A contains, in order, eight spectrin-like repeats, a Dbl/Pleckstrin homology (DH/PH) element, an SH3-like domain, a second DH/PH element, an immunoglobulin domain, and a fibronectin type III domain [3].
Recombinant MTP-1 was catalytically active and digested a range of native and denatured connective tissue substrates, including gelatin, collagen, laminin, and fibronectin [4].
Partial sequence analysis of unc-22 has previously revealed that its encoded polypeptide, named twitchin, consists of a single protein kinase domain and multiple copies of both an immunoglobulin-like domain and a fibronectin type III-like domain [5].
The cDNA sequence of this gene, which has been designated Obscurin-myosin light chain kinase (Obscurin-MLCK), would be predicted to encode for at least 68 immunoglobulin domains, two fibronectin domains, one calcium/calmodulin binding domain, a RhoGTP exchange factor domain, and two serine-threonine kinase domains [6].

Associations of fibronectin with chemical compounds

It consists of multiple copies of both fibronectin III and immunoglobulin C2 domains and, near the C terminus, a protein kinase domain with greatest homology to the catalytic domains of myosin light chain kinases [1].

Other interactions of fibronectin

The purified proteinase, from cultures of L4, has a molecular weight of approximately 46 kDa, functions as an endopeptidase, and digests several native proteins of host origin including fibrinogen and fibronectin [7].

Analytical, diagnostic and therapeutic context of fibronectin

Western blot analysis with monoclonal and polyclonal antibodies that recognize the 30 kDa heparin-binding domain (HBD) and the 45 kDa gelatin-binding domain (GBD) fragments of human fibronectin (Fn) revealed a series of polypeptides of approximately 40, 45 and 55 kDa present in crude cuticle extracts of Meloidogyne javanica 2nd-stage juveniles [8].

References

Protein kinase domain of twitchin has protein kinase activity and an autoinhibitory region. Lei, J., Tang, X., Chambers, T.C., Pohl, J., Benian, G.M. J. Biol. Chem. (1994) [Pubmed]
The conserved immunoglobulin superfamily member SAX-3/Robo directs multiple aspects of axon guidance in C. elegans. Zallen, J.A., Yi, B.A., Bargmann, C.I. Cell (1998) [Pubmed]
UNC-73 activates the Rac GTPase and is required for cell and growth cone migrations in C. elegans. Steven, R., Kubiseski, T.J., Zheng, H., Kulkarni, S., Mancillas, J., Ruiz Morales, A., Hogue, C.W., Pawson, T., Culotti, J. Cell (1998) [Pubmed]
Ancylostoma caninum MTP-1, an astacin-like metalloprotease secreted by infective hookworm larvae, is involved in tissue migration. Williamson, A.L., Lustigman, S., Oksov, Y., Deumic, V., Plieskatt, J., Mendez, S., Zhan, B., Bottazzi, M.E., Hotez, P.J., Loukas, A. Infect. Immun. (2006) [Pubmed]
Additional sequence complexity in the muscle gene, unc-22, and its encoded protein, twitchin, of Caenorhabditis elegans. Benian, G.M., L'Hernault, S.W., Morris, M.E. Genetics (1993) [Pubmed]
Identification, tissue expression and chromosomal localization of human Obscurin-MLCK, a member of the titin and Dbl families of myosin light chain kinases. Russell, M.W., Raeker, M.O., Korytkowski, K.A., Sonneman, K.J. Gene (2002) [Pubmed]
Developmentally regulated zinc metalloproteinases from third- and fourth-stage larvae of the ovine nematode Haemonchus contortus. Gamble, H.R., Fetterer, R.H., Mansfield, L.S. J. Parasitol. (1996) [Pubmed]
The interaction between the gelatin-binding domain of fibronectin and the attachment of Pasteuria penetrans endospores to nematode cuticle. Mohan, S., Fould, S., Davies, K.G. Parasitology (2001) [Pubmed]

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igdb-2	Caenorhabditis elegans
CG17839	Drosophila melanogaster

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