Disease relevance of 16S rRNA

• Genotyping of Francisella tularensis strains by pulsed-field gel electrophoresis, amplified fragment length polymorphism fingerprinting, and 16S rRNA gene sequencing [1].
• Tularemia in a common marmoset (Callithrix jacchus) diagnosed by 16S rRNA sequencing [2].

High impact information on 16S rRNA

• Determination of the sequence of the 16S rRNA gene amplified from the organism and comparison of the sequence to other sequences identified it as a strain of Francisella tularensis, whereas the specific serology was still negative [3].
• The DNA was detected by multiplex PCR targeting the 16S rRNA gene and a 17-kDa lipoprotein gene of F. tularensis [4].
• We report a case of tularemia in a common marmoset (Callithrix jacchus) diagnosed by determination of the isolate's 16S ribosomal RNA (rRNA) gene sequence [2].
• We studied 10 different isolates of F. tularensis from varying hares, one isolate from an Ural owl (Strix uralensis), one vaccine strain, one strain of F. t. biovar japonica, and six isolates from a virulence study of F. tularensis, by biochemical tests and by hybridization experiments with probes complementary to 16S rRNA [5].

Analytical, diagnostic and therapeutic context of 16S rRNA

• The initial survey used 16S rRNA gene primers to detect Francisella species and related organisms by PCR amplification of DNA extracts from environmental samples [6].

References